| Author | Simas, C. J. A. | |
| Author | Silva, D. P. H. | |
| Author | Ponte, C. G. G. | |
| Author | Castello Branco, L. R. R. | |
| Author | Antas, P. R. Z. | |
| Access date | 2018-05-17T12:36:18Z | |
| Available date | 2018-05-17T12:36:18Z | |
| Document date | 2011 | |
| Citation | SIMAS, C. J. A. et al. Patterns of in vitro cell-death, metaloproteinase-9 and pro-inflammatory cytokines in human monocytes induced by the BCG vaccine, Moreau strain. Vaccine, v.29, p.6446-6450, July 2011. | pt_BR |
| ISSN | 0264-410X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26466 | |
| Language | eng | pt_BR |
| Publisher | Elsevier: 12 months | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vacina BCG | pt_BR |
| Subject in Portuguese | Tuberculose | pt_BR |
| Subject in Portuguese | Monócitos | pt_BR |
| Subject in Portuguese | Apoptose | pt_BR |
| Subject in Portuguese | Citocinas | pt_BR |
| Title | Patterns of in vitro cell-death, metaloproteinase-9 and pro-inflammatory cytokines in human monocytes induced by the BCG vaccine, Moreau strain | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vaccine.2011.06.093 | |
| Abstract | Mononuclear cells have been implicated in the primary inflammatory response against mycobacteria. Yet, little is known about the interaction of Mycobacterium bovis bacillus Calmette-Guerin (BCG) with human monocytes. Here, we investigated the potential of BCG Moreau strain to induce in vitro specific cell-death utilizing a flow cytometry approach that revealed an increase in apoptosis events in BCG-stimulated monocytes from healthy adults. We also detected a concomitant release of interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α), but not metalloproteinase (MMP)-9. In addition, annexin V-propidium iodide double staining demonstrated an enhancement of monocytes necrosis, but not apoptosis, following BCG Moreau strain stimulation of umbilical vein cells from naïve, neonate. This pattern was paralleled by different pro-inflammatory cytokine levels, as well as MMP-9 induction when compared to the adults. Our findings support the hypothesis that BCG induces distinct cell-death patterns during the maturation of the immune system and that this pattern might set the stage for a subsequent antimycobacterial immune response that might have profound effects during vaccination. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | BCG vaccine | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | Monocyte | pt_BR |
| Subject | Apoptosis | pt_BR |
| Subject | Cytokine | pt_BR |
| e-ISSN | 1873-2518 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
