Author | Aguiar, Paulo Henrique Palis | |
Author | Melo, Stella Maria Barrouin | |
Author | Franke, Carlos Roberto | |
Author | Santos, Roberto Robson Borges dos | |
Author | Silva, Tânia Maria Correia | |
Author | Mengele Junior, José Orivaldo | |
Author | dosSantos, Washington Luis Conrado | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Access date | 2011-05-11T20:30:25Z | |
Available date | 2011-05-11T20:30:25Z | |
Document date | 2005 | |
Citation | AGUIAR, P. H. P. et al. A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity. Veterinary Journal, v.173, n.1, p.158-166. jan., 2007. | pt_BR |
ISSN | 1090-0233 | |
URI | https://www.arca.fiocruz.br/handle/icict/2652 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.tvjl.2005.08.015 | |
Abstract | This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine
leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear
cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins
with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral
blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and
myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine
splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that
it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of
immune functions in dogs. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Escola de Medicina Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Escola de Medicina Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | Canine | pt_BR |
Subject | Leukocyte | pt_BR |
Subject | Monoclonal antibodies | pt_BR |
Subject | CD45 | pt_BR |
DeCS | Anticorpos Monoclonais | pt_BR |
DeCS | Antígenos | pt_BR |
DeCS | Leucócitos Mononucleares | pt_BR |
DeCS | Proliferação de Células | pt_BR |
DeCS | Distribuição Tecidual | pt_BR |
DeCS | Cães | pt_BR |