| Author | Caldeira, Roberta Lima | |
| Author | Passos, Liana Konovaloffi Jannotti | |
| Author | Carvalho, Omar Dos Santos | |
| Access date | 2018-05-24T13:26:22Z | |
| Available date | 2018-05-24T13:26:22Z | |
| Document date | 2017 | |
| Citation | CALDEIRA, Roberta Lima; PASSOS, Liana Konovaloffi Jannotti; CARVALHO, Omar Dos Santos. Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae). J Trop Med., v. 2017, 8628971, 2017. | pt_BR |
| ISSN | 1687-9686 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26613 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | LS-PCR | pt_BR |
| Subject in Portuguese | S. mansoni | pt_BR |
| Subject in Portuguese | Biomphalaria | pt_BR |
| Subject in Portuguese | Esquistossomose mansoni | pt_BR |
| Title | Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae). | pt_BR |
| Type | Article | |
| DOI | 10.1155/2017/8628971 | |
| Abstract | The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. The assays showed high sensitivity and specificity and could detect S. mansoni DNA when one positive snail was included in a pool of 1,000 negative specimens of Biomphalaria. These molecular approaches can provide a low-cost, effective, and rapid method for detecting the presence of S. mansoni in pooled samples of field-collected Biomphalaria. These assays should aid mapping of transmission sites in endemic areas, especially in low prevalence regions and improve schistosomiasis surveillance. It will be a useful tool to monitor low infection rates of snails in areas where control interventions are leading towards the elimination of schistosomiasis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Moluscário Lobato Paraense. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | LS-PCR | pt_BR |
| Subject | S. mansoni | pt_BR |
| Subject | Biomphalaria | pt_BR |
| Subject | Esquistossomose mansoni | pt_BR |
| Embargo date | 2022-01-01 | |
