| Author | Lucinda, Natália | |
| Author | Figueiredo, Maria Marta | |
| Author | Pessoa, Natália Lima | |
| Author | Santos, Beatriz Senra Álvares da Silva | |
| Author | Lima, Graciela Kunrath | |
| Author | Freitas, Arthur Molinari | |
| Author | Machado, Alexandre Magalhães Vieira | |
| Author | Kroon, Erna Geessien | |
| Author | Antonelli, Lis Ribeiro do Valle | |
| Author | Campos, Marco Antônio | |
| Access date | 2018-05-24T16:20:51Z | |
| Available date | 2018-05-24T16:20:51Z | |
| Document date | 2017 | |
| Citation | LUCINDA, Natália et al. Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B. Virol J., v. 14, n. 1, 37, 2017. | pt_BR |
| ISSN | 1743-422X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26636 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Herpes simplex virus 1 | pt_BR |
| Subject in Portuguese | imunidade inata | pt_BR |
| Title | Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B. | pt_BR |
| Type | Article | |
| DOI | 10.1186/s12985-017-0692-x | |
| Abstract | Background: Herpes simplex virus type 1 (HSV-1) cause not only mild symptoms but also blindness and encephalitis. It was previously shown that the immune response against HSV-1 occurs mainly in the trigeminal ganglia (TG) and that Toll-like receptors 2 and 9 (TLR2/9) are important in mediating this response. It was also demonstrated that iNOS (nitric oxide synthase) and interleukin 1 beta (IL-1β) play an essential role in the defense against HSV-1 infection. Importantly, the present work aimed to identify the primary cells responsible for iNOS and IL-1β production and search for other important molecules and cells that might or might not depend on TLR2/9 receptors to mediate the immune response against HSV-1.
Methods: C57BL/6 (wild type, WT) and TLR2/9−/− mice were infected by the intranasal route with HSV-1 (1 × 106 p.f.u.). Cells were obtained from the TG and spleen tissues and the profile of immune cells was determined by flow cytometry in infected and mock infected WT and knockout mice. The percentage of cells producing iNOS, IL-1β, granzyme B and perforin was also determined by flow cytometry. Chemokine monocyte chemoattractant protein-1 (MCP1) was measured by Cytometric Bead Array (CBA) in the TG, spleen and lung. Expression of type I interferons (IFNs), interleukins (IL) 5 and 10, IL-1β and granzyme B were quantified by real time PCR.
Results: The results indicate that dendritic cells (DCs) and monocytes/macrophages (Mo/Mϕ) were the main sources of IL-1β and iNOS, respectively, which, together with type I IFNs, were essential for the immune response against HSV-1. Additionally, we showed that granzyme B produced by CD8+ T and NK lymphocytes and MCP-1 were also important for this immune response. Moreover, our data indicate that the robust production of MCP-1 and granzyme B is either TLR-independent or down regulated by TLRs and occurs in the TG of TLR2/9−/− infected mice.
Conclusion: Taken together, our data provide strong evidence that the responses mediated by DCs, Mo/Mϕ, NK and CD8+ T lymphocytes through IL-1β, iNOS and granzyme B production, respectively, together with the production of type I IFN early in the infection, are crucial to host defense against HSV-1. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia e Imunologia Parasitária. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Herpes simplex virus 1 | pt_BR |
| Subject | Innate immunity | pt_BR |
| Subject | Dendritic cells | pt_BR |
| Subject | Macrophages | pt_BR |
| Subject | CD8+ T lymphocytes | pt_BR |
| Subject | TLRs | pt_BR |
| Subject | Murine model | pt_BR |
| Subject | Neuropathogenesis | pt_BR |
| Subject | Encephalitis | pt_BR |
| Embargo date | 2023-01-01 | |
