| Author | Reis, Jordana Grazziela Alves Coelho dos | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Xavier, Marcelo Antônio Pascoal | |
| Author | Gomes, Matheus de Souza | |
| Author | Amaral, Laurence Rodrigues do | |
| Author | Cardoso, Ludmila Melo | |
| Author | Gonçalves, Juan Jonathan | |
| Author | Ribeiro, Ágata Lopes | |
| Author | Starling, Ana Lúcia Borges | |
| Author | Ribas, João Gabriel | |
| Author | Gonçalves, Denise Utsch | |
| Author | Proietti, Anna Bárbarade Freitas Carneiro | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Martins Filho, Olindo Assis | |
| Author | Grupo Interdisciplinar de Pesquisa em HTLV-1 | |
| Access date | 2018-05-24T18:07:04Z | |
| Available date | 2018-05-24T18:07:04Z | |
| Document date | 2017 | |
| Citation | COELHO, Jordana Grazziela Alves Coelho dos et al. Flow cytometric-based protocols for assessing anti-MT-2 IgG1 reactivity: High-dimensional data handling to define predictors for clinical follow-up of Human T-cell Leukemia virus type-1 infection. Journal of Immunological Methods, v. 444, p. 36-46, 2017. | pt_BR |
| ISSN | 0022-1759 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26641 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | HTLV-1 | pt_BR |
| Subject in Portuguese | HAM/TSP | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Acompanhamento clinico | pt_BR |
| Subject in Portuguese | Citometria de fluxo | pt_BR |
| Title | Flow cytometric-based protocols for assessing anti-MT-2 IgG1 reactivity: High-dimensional data handling to define predictors for clinical follow-up of Human T-cell Leukemia virus type-1 infection | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jim.2017.02.006 | |
| Abstract | The present work provides an innovative methodological approach to assess the anti-HTLV-1 IgG1 reactivity with practical application in clinical laboratory. Serum from non-infected healthy controls (NI) and HTLV-1-infected patients, categorized as asymptomatic (AS), putatively progressing to HTLV-1 associated myelopathy/tropical spastic paraparesis - HAM/TSP (pHAM) or with clinical diagnosis of HAM/TSP (HT) were assayed in two-parallel flow cytometry platforms, referred as: Fix and Fix&Perm protocols. Operating-characteristics analysis indicated that a single pair of attributes (“serum dilution/cut-off”) for Fix and Fix&Perm protocols presented excellent performance for the diagnosis of HTLV-1 infection. Conversely, Fix and Fix&Perm protocols displayed weak/moderate overall performances when applied with prognosis purposes of HTLV-1 infection. A panoramic snapshot provided by the reactivity boards revealed clearly the higher sensitivity of Fix&Perm protocol for detecting seropositivity for HT, suggesting that stepwise combinatory criteria would improve the global performance of using a single pair of attributes. Three data mining strategies were tested, including endpoint titer analysis, heatmap assemblage and decision tree analysis. Bi-dimensional heatmap analysis demonstrated that, while the clustering profile of NI vs HTLV-1+ revealed segregation in opposite poles, AS vs HT presented discrete segregation but still displaying an intertwined distribution pattern. The combination of methods for segregating AS from HT displayed a moderate but superior global accuracy (85.7%; LOOCV = 71.4%). The comprehensive data analysis support that the combination of methods have improved the performance to the differential diagnosis of AS and HT, with direct association with laboratorial records, including serum cytokine levels and proviral load. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Faculdade de Computação. Instituto de Genética e Bioquímica. Laboratório de Bioinformática e Análise Molecular. Patos de Minas, MG, Brasil. | pt_BR |
| Affilliation | Faculdade de Computação. Instituto de Genética e Bioquímica. Laboratório de Bioinformática e Análise Molecular. Patos de Minas, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Centro de Hematologia e Hemoterapia de Minas Gerais. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Hospital Sarah Kubitschek. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Centro de Hematologia e Hemoterapia de Minas Gerais. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | HTLV-1 | pt_BR |
| Subject | HAM/TSP | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Clinical follow-up | pt_BR |
| Subject | Flow cytometry | pt_BR |
| Embargo date | 2022-01-01 | |
