| Author | Oliveira, Edson R. A. | |
| Author | Póvoa, Tiago F. | |
| Author | Nuovo, Gerard J. | |
| Author | Allonso, Diego | |
| Author | Salomão, Natália G. | |
| Author | Oliveira, Carlos A. Basílio de | |
| Author | Geraldo, Luiz H. M. | |
| Author | Fonseca, Celina G. | |
| Author | Lima, Flávia R. S. | |
| Author | Borges, Ronaldo Mohana | |
| Author | Paes, Marciano V. | |
| Access date | 2018-06-01T20:18:20Z | |
| Available date | 2018-06-01T20:18:20Z | |
| Document date | 2017 | |
| Citation | OLIVEIRA, Edson R. A. et al. Dengue fatal cases present virusspecific HMGB1 response in peripheral organs. Scientifc Reports, v.7:16011, 14p, Nov. 2017. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26700 | |
| Language | eng | pt_BR |
| Publisher | Nature Publishing Group | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Dengue | pt_BR |
| Subject in Portuguese | Casos fatáis | pt_BR |
| Subject in Portuguese | vírus HMGB1 | pt_BR |
| Subject in Portuguese | órgãos periféricos | pt_BR |
| Title | Dengue fatal cases present virus-specific HMGB1 response in peripheral organs | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-017-16197-5 | |
| Abstract | Dengue is an important infectious disease that presents high incidence and yields a relevant number of fatal cases (about 20,000) every year worldwide. Despite its epidemiological relevance, there are many knowledge gaps concerning dengue pathogenesis, especially with regards to the circumstances that drive a mild clinical course to a severe disease. In this work, we investigated the participation of high mobility group box 1 (HMGB1), an important modulator of inflammation, in dengue fatal cases. Histopathological and ultrastructural analyses revealed that liver, lung and heart post-mortem samples were marked by tissue abnormalities, such as necrosis and apoptotic cell death. These observations go in line with an HMGB1-mediated response and raised concerns regarding the participation of this cytokine in promoting/perpetuating inflammation in severe dengue. Further experiments of immunohistochemistry (IHC) showed increased expression of cytoplasmic HMGB1 in dengue-extracted tissues when compared to non-dengue controls. Co-staining of DENV RNA and HMGB1 in the host cell cytoplasm, as found by in situ hybridization and IHC, confirmed the virus specific induction of the HMGB1-mediated response in these peripheral tissues. This report brings the first in-situ evidence of the participation of HMGB1 in severe dengue and highlights novel considerations in the development of dengue immunopathogenesis. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química Orgânica. Laboratório de Modelagem Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto de Criminalística. Tocantins, Brasil. | pt_BR |
| Affilliation | Ohio State University Comprehensive Cancer Center. Columbus, Ohio, USA / Phylogeny Inc. Powell, Ohio, USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Genômica Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Gaffrée Guinle. Anatomia Patológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Biologia das Células Gliais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Biologia das Células Gliais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Biologia das Células Gliais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Genômica Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Dengue | pt_BR |
| Subject | fatal cases | pt_BR |
| Subject | virus specific HMGB1 | pt_BR |
| Subject | peripheral organs | pt_BR |
| e-ISSN | 2045-2322 | |
