| Author | Wowk, Pryscilla Fanini | |
| Author | Franco, Luıs Henrique | |
| Author | Fonseca, Denise Morais da | |
| Author | Paula, Marina Oliveira | |
| Author | Vianna, Elcio dos Santos Oliveira | |
| Author | Wendling, Ana Paula | |
| Author | Augusto, Valeria Maria | |
| Author | Santos, Silvana Maria Eloi | |
| Author | Carvalho, Andrea Teixeira de | |
| Author | Silva, Flavia Dias Coelho | |
| Author | Vinhas, Solange Alves | |
| Author | Martins Filho, Olindo Assis | |
| Author | Palaci, Moises | |
| Author | Silva, Celio Lopes | |
| Author | Bonato, Vania Luiza Deperon | |
| Access date | 2018-06-06T17:17:20Z | |
| Available date | 2018-06-06T17:17:20Z | |
| Document date | 2017 | |
| Citation | WOWK, Pryscilla Fanini et al. Mycobacterial Hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients. Hum Vaccin Immunother., v. 13, n. 5, p. 1040-1050, 2017. | pt_BR |
| ISSN | 2164-5515 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26748 | |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | IFN-γ | pt_BR |
| Subject in Portuguese | IL-10 | pt_BR |
| Subject in Portuguese | tuberculose | pt_BR |
| Subject in Portuguese | vacina | pt_BR |
| Title | Mycobacterial Hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients | pt_BR |
| Type | Article | |
| DOI | 10.1080/21645515.2016.1264547 | |
| Abstract | Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4+ or CD8+cell were assessed by flow cytometry. The CD4+ cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8+ cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN-gamma+ cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Bioquimica e Imunologia. Ribeirão Preto, SP, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Bioquimica e Imunologia. Ribeirão Preto, SP, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Bioquimica e Imunologia. Ribeirão Preto, SP, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Bioquimica e Imunologia. Ribeirão Preto, SP, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Divisão de Respiração. Ribeirão Preto, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Biomarcados e Monitoramento de Diagnostico. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Biomarcados e Monitoramento de Diagnostico. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espirito Santo. Núcleo de Doenças Infecciosas. Laboratório de Mycobacteriologia. Vitoria, ES, Brasil | pt_BR |
| Affilliation | Universidade Federal do Espirito Santo. Núcleo de Doenças Infecciosas. Laboratório de Mycobacteriologia. Vitoria, ES, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Biomarcados e Monitoramento de Diagnostico. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espirito Santo. Núcleo de Doenças Infecciosas. Laboratório de Mycobacteriologia. Vitoria, ES, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Bioquimica e Imunologia. Ribeirão Preto, SP, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departmento de Bioquimica e Imunologia. Ribeirão Preto, SP, Brasil | pt_BR |
| Subject | IFN-γ | pt_BR |
| Subject | IL-10 | pt_BR |
| Subject | tuberculosis | pt_BR |
| Subject | vaccine | pt_BR |
| Embargo date | 2024-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
