| Author | Paiva, Magna Cristina de | |
| Author | Guimarães, Mariana de Paula Reis | |
| Author | Costa, Patrícia Silva | |
| Author | Dias, Marcela F. | |
| Author | Bleicher, Lucas | |
| Author | Scholte, Larissa Lopes Silva | |
| Author | Drummond, Regina Maria Nardi | |
| Author | Nascimento, Andrea Maria Amaral | |
| Access date | 2018-06-06T18:08:32Z | |
| Available date | 2018-06-06T18:08:32Z | |
| Document date | 2017 | |
| Citation | PAIVA, Magna Cristina de et al. Identification of new bacteria harboring qnrS and aac(6')-Ib/cr and mutations possibly involved in fluoroquinolone resistance in raw sewage and activated sludge samples from a full-scale WWTP. Water Res., v. 110, p.27-37, 2017. | pt_BR |
| ISSN | 0043-1354 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26751 | |
| Language | eng | pt_BR |
| Publisher | Pergamon Press | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Bacteria | pt_BR |
| Subject in Portuguese | Quinolone | pt_BR |
| Subject in Portuguese | qnrS | pt_BR |
| Subject in Portuguese | aac(6′)Ib | pt_BR |
| Title | Identification of new bacteria harboring qnrS and aac(6')-Ib/cr and mutations possibly involved in fluoroquinolone resistance in raw sewage and activated sludge samples from a full-scale WWTP | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.watres.2016.11.056 | |
| Abstract | Wastewater treatment plants (WWTPs) harbor bacteria and antimicrobial resistance genes, favoring gene exchange events and resistance dissemination. Here, a culture-based and metagenomic survey of qnrA, qnrB, qnrS, and aac(6′)-Ib genes from raw sewage (RS) and activated sludge (AS) of a full-scale municipal WWTP was performed. A total of 96 bacterial isolates were recovered from nalidixic acid-enrichment cultures. Bacteria harboring the aac(6′)-Ib gene predominated in RS, whereas qnrS-positive isolates were specific to AS. Novel qnrS- and aac(6′)-Ib-cr positive species were identified: Morganella morganii, Providencia rettgeri, and Pseudomonas guangdongensis (qnrS), and Alcaligenes faecalis and P. rettgeri (aac(6′)-Ib-cr). Analysis of qnrS and aac(6′)-Ib sequences from isolates and clone libraries suggested that the diversity of qnrS is wider than that of aac(6′)-Ib. A large number of amino acid mutations were observed in the QnrS and AAC(6′)-Ib proteins at previously undetected positions, whose structural implications are not clear. An accumulation of mutations at the C72, Q73, L74, A75 and M76 positions of QnrS, and D181 of AAC(6′)-Ib might be important for resistance. These findings add significant information on bacteria harboring qnrS and aac(6′)-Ib genes, and the presence of novel mutations that may eventually emerge in clinical isolates. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Universidade Federal de São João del Rei. Divinópolis, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Bacteria | pt_BR |
| Subject | Quinolone | pt_BR |
| Subject | qnrS | pt_BR |
| Subject | aac(6′)Ib | pt_BR |
| Subject | aac(6′)Ib-cr | pt_BR |
| Subject | Raw sewage | pt_BR |
| Subject | Activated sludge | pt_BR |
| Embargo date | 2026-01-01 | |
| xmlui.metadata.dc.subject.ods | 06 Água potável e saneamento | |
