| Author | Cunha, Joana Paixão Monteiro | |
| Author | Souza, Juliana Sacramento Mota de | |
| Author | Diaz, Ricardo Sobhie | |
| Author | Rego, Filipe Ferreira de Almeida | |
| Author | Andrade, E | |
| Author | Silva, Márcio de Oliveira | |
| Author | Marta, Giovanetti | |
| Author | Oliveira, T. de | |
| Author | Oliveira, A | |
| Author | Alves, Carlos Roberto Brites | |
| Author | Alcantara, Luiz Carlos Júnior | |
| Access date | 2018-06-07T14:08:10Z | |
| Available date | 2018-06-07T14:08:10Z | |
| Document date | 2017 | |
| Citation | CUNHA, J. P. M. et al. HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals. Journal of AIDS and HIV Infections, v.3, n. 1, p. 103, 2017. | pt_BR |
| ISSN | 2454-499X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26766 | |
| Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Annex Publishers | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Genotipagem | pt_BR |
| Subject in Portuguese | Mutação | pt_BR |
| Subject in Portuguese | Antirretroviral | pt_BR |
| Subject in Portuguese | Subtipos | pt_BR |
| Subject in Portuguese | HIV | pt_BR |
| Subject in Portuguese | Resistência | pt_BR |
| Subject in Portuguese | Introdução à Prevalência | pt_BR |
| Title | HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals | pt_BR |
| Type | Article | pt_BR |
| Abstract | 499XThe distribution of different human immunodeficiency virus type 1 (HIV-1) genotypes and the prevalence of transmitted drug resistance (TDR) mutations vary greatly across different Brazilian regions. This study aimed to describe the HIV-1 molecular diversity and TDR prevalence among treatment-naïve HIV-1 infected individuals in an urban area of Northeastern Brazil. DNA samples from 97 infected individuals were obtained and pol sequences were generated by Polimerase Chain Reaction (PCR) and direct sequencing. Bioinformatics tools were used to identify the presence of associated mutations with drug resistance, to reconstruct the phylogeny and to detect recombination. The median age and estimated time of HIV-1 diagnosis were 34 years and 12 months, respectively and 61% of the patients were male. The main exposure category was sexual (87.5%) and the median TCD4+ lymphocyte counts and viral load were 438 cells/mm3 and 4.4 log10, respectively. The overall TDR prevalence was 5.1% while within each drug class (Non-nucleoside reverse-transcriptase inhibitors - NNRTIs, Nucleoside analog reverse-transcriptase inhibitors - NRTIs and Protease inhibitors - PIs) this rate was 2.1% individually. These TDR mutations confer intermediate to high-level resistance to different drugs that are used as first-line treatment options, which reinforce the importance of genotype testing prior to treatment initia-tion. Phylogenetic analysis showed that 73.2% of the samples were subtype B, 14.4% were subtype F, 4.1% were subtype C and 8.3% were BF recombinants. Our data indicate that the genotypic profile of HIV-1 strains circulating in Bahia is diversified and show an increase of subtype C prevalence in Northeast Brazil | pt_BR |
| Affilliation | Federal University of Bahia. Core Bioinformatics Department Bioformatics. Salvador, BA, Brazil / Bahia School of Medicine and Public Health. Bahia Foundation for the Development of Sciences. Salvador, BA, Brazil | pt_BR |
| Affilliation | Federal University of Bahia. Core Bioinformatics Department Bioformatics. Salvador, BA, Brazil | pt_BR |
| Affilliation | Federal University of São Paulo. Department of Medicine. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of KwaZulu-Natal. Africa Centre for Health and Population Studies. Durban, South Africa | pt_BR |
| Affilliation | State Center Specialized in Diagnosis. Assistance and Research. Salvador, BA, Brazil | pt_BR |
| Affilliation | Federal University of Bahia. Professor Edgard Santos University Hospital Complex. Laboratory of Infectology Research. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Subject | Genotyping | pt_BR |
| Subject | Mutation | pt_BR |
| Subject | Antiretroviral | pt_BR |
| Subject | Subtypes | pt_BR |
| Subject | HIV | pt_BR |
| Subject | Resistance | pt_BR |
| Subject | Prevalence Introduction | pt_BR |
