| Author | Medeiros, Nayara Ingrid de | |
| Author | Gusmão, Rafaelle Christine Gomes Fares | |
| Author | Franco, Eliza P. | |
| Author | Sousa, Giovane R. | |
| Author | Mattos, Rafael Teixeira de | |
| Author | Chaves, Ana Thereza | |
| Author | Nunes, Maria do Carmo Pereira | |
| Author | Dutra, Walderez Ornelas | |
| Author | Oliveira, Rodrigo Correa de | |
| Author | Rocha, Manoel Otávio da Costa | |
| Author | Estanislau, Juliana de Assis Silva Gomes | |
| Access date | 2018-06-07T18:14:28Z | |
| Available date | 2018-06-07T18:14:28Z | |
| Document date | 2017 | |
| Citation | MEDEIROS, Nayara Ingrid de et al. Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. PLoS Negl Trop Dis., v. 11, n. 1, e0005284, 2017. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26785 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Doenças cardiacas | pt_BR |
| Title | Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease. | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0005284 | |
| Abstract | Dilated cardiomyopathy, the most severe manifestation in chronic phase of Chagas disease, affects about 30% of patients and is characterized by myocardial dysfunction and interstitial fibrosis due to extracellular matrix (ECM) remodeling. ECM remodeling is regulated by proteolytic enzymes such as matrix metalloproteinases (MMPs) and cytokines produced by immune cells, including phagocytes. We evaluated by flow cytometry the expression of MMP-2, MMP-9, IL-1β, TNF-α, TGF-β and IL-10 by neutrophils and monocytes from patients with indeterminate (IND) and cardiac (CARD) clinical forms of Chagas disease and non-infected individuals (NI), before and after in vitro stimulation with Trypanosoma cruzi antigens. Our results showed an important contribution of neutrophils for MMPs production, while monocytes seemed to be involved in cytokine production. The results showed that neutrophils and monocytes from IND and CARD patients had higher intracellular levels of MMP-2 and MMP-9 than NI individuals. On the other hand, T. cruzi derived-antigens promote a differential expression of MMP-2 and MMP-9 in patients with Chagas disease and may regulate MMPs expression in neutrophils and monocytes, mainly when a cardiac alteration is not present. Our data also showed that in the presence of T. cruzi derived-antigens the production of cytokines by neutrophils and monocytes, but mainly by monocytes, may be intensified. Correlation analysis demonstrated that MMP-2 had a positive correlation with IL-10 and a negative correlation with IL-1β, whereas MMP-9 showed a negative correlation with IL-10. We also observed that IND patients presented a greater percentage of high producer cells of regulatory molecules when compared to CARD patients, indicating a different pattern in the immune response. Our data suggest that MMPs and cytokines produced by neutrophils and monocytes are important contributors for cardiac remodeling and may be an interesting target for new biomarker research. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | United States Food and Drug Administration. Center for Biologics Evaluation and Research - Silver Spring. Maryland, United States of America/Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciência e Tecnologia em Doenças Topicais. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciência e Tecnologia em Doenças Topicais. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | cardiac diseases | pt_BR |
| Embargo date | 2020-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
