Author | Campos, Liliane Cunha | |
Author | Silva, Juliana de Oliveira | |
Author | Souza, Cristina Maria de | |
Author | Campos, Cecília Bonolo de | |
Author | Gamba, Conrado de Oliveira | |
Author | Damasceno, Karine Araújo | |
Author | Horta, Rodrigo dos Santos | |
Author | Lavalle, Gleidice Eunice | |
Author | Cassali, Geovanni Dantas | |
Access date | 2018-06-08T13:12:24Z | |
Available date | 2018-06-08T13:12:24Z | |
Document date | 2013 | |
Citation | CAMPOS, L. C. et al. Adenocarcinoma papilar de seio nasal em cão: Abordagem histomorfológica, imunofenotípica e terapêutica. Acta Scientiae Veterinariae, v. 41, Suppl 1, pub. 16, 2013. | pt_BR |
ISSN | 1678-0345 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/26795 | |
Description | Damasceno, Karine Araújo. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Language | por | pt_BR |
Publisher | Universidade Federal do Rio Grande do Sul. Faculdade de Veterinaria | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Imuno-histoquímica | pt_BR |
Subject in Portuguese | Canino | pt_BR |
Subject in Portuguese | Trato respiratório | pt_BR |
Subject in Portuguese | Neoplasia | pt_BR |
Title | Adenocarcinoma papilar de seio nasal em cão: Abordagem histomorfológica, imunofenotípica e terapêutica | pt_BR |
Alternative title | Papillary Adenocarcinoma of Nasal Sinus in a Dog: Approach Histomorphologic, Immunophenotypic, and Therapeutic | pt_BR |
Type | Article | pt_BR |
Abstract | Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by
slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary
pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas
are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the
use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.
Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region,
was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish
color, and some fragments associated with more fi rm tissues like cartilage and bone tissue. Tumor specimens were collected, fi xed in
10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 μm histological sections were obtained and stained with
HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance
HRP. CKAE1/AE3, CK34βE12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3 antibody a
qualitative method was used and neoplasia were classifi ed as negative (-) or positive (+) and for CK34βE12 was used semiquantitative
evaluation and neoplasms were classifi ed as negative (-), positive with focal staining (+) and positive with diffuse staining (++). The
graduation for HER-2 expression was (+) for 4 (++++), were 4 (++++) was the most intensity membrane staining. For Cox-2 the system
used was semiquantitative and the distribution was scored by estimating the percentage of tumor cells staining. Intensity was estimated
on a scale from 0 (absent) to 12 (strong). The proliferative index was calculated by counting nuclei positive for Ki-67 (anti-MIB-1) in
a total of 1000 neoplastic cells. Microscopically, was observed a proliferation of epithelial cells in papillary arrangement with delicate
connective axis of support and areas of stromal invasion, and the tumor received the diagnosis of papillary adenocarcinoma of nasal
sinus. Immunohistochemical analysis revealed strong staining for Cox-2 (score 12), CKAE1/AE3 positivity and CK34βE12 (+ + +)
and negative for Her-2 (+). Loss of E-cadherin expression was observed and SMA allowed the visualization of areas of basal membrane
rupture. The rate of proliferation was evaluated by means of the expression of MIB-1 was high (16%). Chemotherapy was performed
with carboplatin and gemcitabine. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Subject | Immunohistochemical | pt_BR |
Subject | Canine | pt_BR |
Subject | Respiratory tract | pt_BR |
Subject | Neoplasia | pt_BR |