| Author | Botan, Valéria | |
| Author | Borges, Tatiana Karla dos Santos | |
| Author | Alves, Érica Alessandra Rocha | |
| Author | Couto, Shirley Claudino Pereira | |
| Author | Seidler, Heinrich Bender Kohnert | |
| Author | Junqueira, Maria Imaculada Muniz | |
| Access date | 2018-06-12T12:23:16Z | |
| Available date | 2018-06-12T12:23:16Z | |
| Document date | 2017 | |
| Citation | BOTAN, Valéria et al. Enhanced activation of eosinophils in peripheral blood and implications for eosinophilic esophagitis diagnosis. J Gastroenterol Hepatol., v. 32, n. 7, p. 1318-1327, 2017. | pt_BR |
| ISSN | 0815-9319 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26815 | |
| Language | eng | pt_BR |
| Publisher | Asian Pacific Association of Gastroenterology | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | cyclooxygenase-2 | pt_BR |
| Subject in Portuguese | eicosanóides | pt_BR |
| Subject in Portuguese | esofagite eosinofílica | pt_BR |
| Subject in Portuguese | eosinófilos | pt_BR |
| Title | Enhanced activation of eosinophils in peripheral blood and implications for eosinophilic esophagitis diagnosis. | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1111/jgh.13710 | |
| Abstract | Background and Aim: Eosinophils are markers of the eosinophilic esophagitis (EoE) disease, and this work aimed to assess whether activation of eosinophils could be a noninvasive test to contribute for EoE diagnosis.
Methods: The activation state of peripheral blood eosinophils in EoE patients and control subjects was assessed based on the morphological aspects of the eosinophil after adherence to slide. Cyclooxygenase-2 and 5-lipoxygenase expressions were evaluated by means of immunofluorescence microscopy to verify if and which eicosanoid pathway is triggered in eosinophils in blood in EoE.
Results: The eosinophils of patients with EoE were significantly more activated than those of control individuals. The lowest percentage of normal eosinophils for control subjects was 40%, while the highest percentage of eosinophils of normal aspect for patients with EoE was 32%. Considering 36% as a cutoff for normal eosinophils, this value differentiated all individuals with EoE from individuals without the disease with a sensitivity of 100%, considering the diagnosis of EoE as currently defined. Eosinophils of EoE patients showed higher expression of cyclooxygenase-2 than those of control subjects.
Conclusions: The quantification of morphological changes in eosinophils is a feasible,easy, and reliable manner to identify EoE patients. Therefore, patients with symptoms of esophageal dysfunction showing higher than 36% activated eosinophils in peripheral blood could be a useful way to help definition and diagnostic criterion for EoE. | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratorio de Imunologia Celular. Brasilia, DF, Brasil/Clinica Alergya. Brasilia, DF, Brasil | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratorio de Imunologia Celular. Brasilia, DF, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Imunologia Molecular Celular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratorio de Imunologia Celular. Brasilia, DF, Brasil | pt_BR |
| Affilliation | Laboratorio Brasiliensis. Brasilia, DF, Brasil/Universidade Catolica de Brasília. Brasília, DF, Brasil | pt_BR |
| Affilliation | Universidade de Brasilia. Faculdade de Medicina. Laboratorio de Imunologia Celular. Brasilia, DF, Brasil | pt_BR |
| Subject | cyclooxygenase-2 | pt_BR |
| Subject | eicosanoids | pt_BR |
| Subject | eosinophilic esophagitis | pt_BR |
| Subject | eosinophils | pt_BR |
| Embargo date | 2060-01-01 | |
