| Autor | Luthold, Renata Vidonscky | |
| Autor | Fernandes, Gabriel da Rocha | |
| Autor | Moraes, Ana Carolina Franco de | |
| Autor | Folchetti, Luciana Gavilan Dias | |
| Autor | Vivolo, Sandra Roberta Gouvea Ferreira | |
| Data de acesso | 2018-06-12T15:53:15Z | |
| Data de disponibilização | 2018-06-12T15:53:15Z | |
| Data do publicação | 2017 | |
| Citação | LUTHOLD, Renata Vidonscky et al. Gut microbiota interactions with the immunomodulatory role of vitamin D in normal individuals. Metabolism, v.69, p. 76-86, 2017. | pt_BR |
| ISSN | 0026-0495 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26838 | |
| Idioma | eng | pt_BR |
| Editor | Elsevier | pt_BR |
| Direito Autoral | restricted access | pt_BR |
| Palavras-chave | Bacterias gram negativa | pt_BR |
| Título | Gut microbiota interactions with the immunomodulatory role of vitamin D in normal individuals. | pt_BR |
| Tipo do documento | Article | |
| DOI | 10.1016/j.metabol.2017.01.007 | |
| Resumo em Inglês | BACKGROUND: Due to immunomodulatory properties, vitamin D status has been implicated in several diseases beyond the skeletal disorders. There is evidence that its deficiency deteriorates the gut barrier favoring translocation of endotoxins into the circulation and systemic inflammation. Few studies investigated whether the relationship between vitamin D status and metabolic disorders would be mediated by the gut microbiota composition.
OBJECTIVE: We examined the association between vitamin D intake and circulating levels of 25(OH)D with gut microbiota composition, inflammatory markers and biochemical profile in healthy individuals.
METHODS: In this cross-sectional analysis, 150 young healthy adults were stratified into tertiles of intake and concentrations of vitamin D and their clinical and inflammatory profiles were compared. The DESeq2 was used for comparisons of microbiota composition and the log2 fold changes (log2FC) represented the comparison against the reference level. The association between 25(OH)D and fecal microbiota (16S rRNA sequencing, V4 region) was tested by multiple linear regression.
RESULTS: Vitamin D intake was associated with its concentration (r=0.220, p=0.008). There were no significant differences in clinical and inflammatory variables across tertiles of intake. However, lipopolysaccharides increased with the reduction of 25(OH)D (p-trend <0.05). Prevotella was more abundant (log2FC 1.67, p<0.01), while Haemophilus and Veillonella were less abundant (log2FC -2.92 and -1.46, p<0.01, respectively) in the subset with the highest vitamin D intake (reference) than that observed in the other subset (first plus second tertiles). PCR (r=-0.170, p=0.039), E-selectin (r=-0.220, p=0.007) and abundances of Coprococcus (r=-0.215, p=0.008) and Bifdobacterium (r=-0.269, p=0.001) were inversely correlated with 25(OH)D. After adjusting for age, sex, season and BMI, 25(OH)D maintained inversely associated with Coprococcus (β=-9.414, p=0.045) and Bifdobacterium (β=-1.881, p=0.051), but significance disappeared following the addition of inflammatory markers in the regression models.
CONCLUSION: The role of vitamin D in the maintenance of immune homeostasis seems to occur in part by interacting with the gut microbiota. The attenuation of association of bacterial genera by inflammatory markers suggests that inflammation participate in part in the relationship between the gut microbiota and vitamin D concentration. Studies with appropriate design are necessary to address hypothesis raised in the current study. | pt_BR |
| Afiliação | Universidade de São Paulo. Faculdade de Saude Publica. Departamento de Nutrição. São Paulo, SP, Brazil | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Universidade de São Paulo. Faculdade de Saude Publica. Departamento de Epidemiologia. São Paulo, SP, Brazil | pt_BR |
| Afiliação | Universidade de São Paulo. Faculdade de Saude Publica. Departamento de Nutrição. São Paulo, SP, Brazil | pt_BR |
| Afiliação | Universidade de São Paulo. Faculdade de Saude Publica. Departamento de Epidemiologia. São Paulo, SP, Brazil | pt_BR |
| Palavras-chave em inglês | Gram-negative bacteria | pt_BR |
| Palavras-chave em inglês | Gut microbiota | pt_BR |
| Palavras-chave em inglês | Inflammation | pt_BR |
| Palavras-chave em inglês | Vitamin D | pt_BR |
| Data de embargo | 2023-01-01 | |
