| Author | Nogueira, Rita de Maria Seabra | |
| Author | Silva, Arannadia Barbosa | |
| Author | Sato, Tayra Pereira | |
| Author | Sá, Joicy Cortez de | |
| Author | Santos, Ana Clara Gomes dos | |
| Author | Amorim Filho, Edvaldo Franco | |
| Author | Vale, Tássia Lopes do | |
| Author | Gazeta, Gilberto Salles | |
| Access date | 2018-06-26T11:53:12Z | |
| Available date | 2018-06-26T11:53:12Z | |
| Document date | 2017 | |
| Citation | NOGUEIRA, Rita de Maria Seabra; et al. Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil. Pesquisa Veterinária Brasileira, v.37, n.12, p.1416-1422, 2017. | pt_BR |
| ISSN | 0100-736X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27094 | |
| Language | eng | pt_BR |
| Publisher | Colégio Brasileiro de Patologia Animal | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Cavalos | pt_BR |
| Subject in Portuguese | Anaplasma phagocytophilum | pt_BR |
| Subject in Portuguese | Carrapatos | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Subject in Portuguese | Babesia caballi | pt_BR |
| Subject in Portuguese | Theileria equi | pt_BR |
| Subject in Portuguese | Doença transmitida por vetor | pt_BR |
| Title | Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0100-736X2017001200010 | |
| Abstract | Equine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans
Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease.
In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis,
causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In
this sense, blood samples and ticks were collected from 97 horses raised in the microregion
of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect
Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain
Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma
phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi,
B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed
13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of
170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense
sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170)
and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All
samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed
when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn
analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA
gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the
obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that
these pathogens occur and are circulating in the studied area. | pt_BR |
| Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ. Brasil / Universidade Federal de Juiz de Fora. Programa de Pós-Graduação em Comportamento e Biologia Animal. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Vector-borne disease | pt_BR |
| Subject | Theileria equi | pt_BR |
| Subject | Babesia caballi | pt_BR |
| Subject | Anaplasma phagocytophilum | pt_BR |
| Subject | horses | pt_BR |
| Subject | ticks | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1678-5150 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
