| Author | Silva, Rodrigo Nunes Rodrigues da | |
| Author | Soares, Isabela Ferreira | |
| Author | Lopez-Camacho, Cesar | |
| Author | Silva, João Hermínio Martins da | |
| Author | Silva, Daiana de Souza Perce da | |
| Author | Teva, Antonio | |
| Author | Franco, Antônia Maria Ramos | |
| Author | Pinheiro, Francimeire Gomes | |
| Author | Chaves, Lana Bitencourt | |
| Author | Pratt-Riccio, Lilian Rose | |
| Author | Reyes-Sandoval, Arturo | |
| Author | Banic, Dalma Maria | |
| Author | Lima Junior, Josué da Costa | |
| Access date | 2018-06-26T12:35:16Z | |
| Available date | 2018-06-26T12:35:16Z | |
| Document date | 2017 | |
| Citation | SILVA, Rodrigo Nunes Rodrigues da; et al. Plasmodium vivax cell-Traversal Protein for Ookinetes and sporozoites: naturally acquired humoral immune response and B-cell epitope Mapping in Brazilian amazon inhabitants. Frontiers in Immunology, v.8, Article 77, 13p, Feb. 2017. | pt_BR |
| ISSN | 1664-3224 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27097 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Malária | pt_BR |
| Subject in Portuguese | Vacinas | pt_BR |
| Subject in Portuguese | Plasmodium vivax | pt_BR |
| Subject in Portuguese | mapeamento de epitopos | pt_BR |
| Subject in Portuguese | previsão de epítopo | pt_BR |
| Subject in Portuguese | Amazônia Brasileira | pt_BR |
| Title | Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants | pt_BR |
| Type | Article | |
| DOI | 10.3389/fimmu.2017.00077 | |
| Abstract | The cell-traversal protein for ookinetes and sporozoites (CelTOS), a highly conserved antigen involved in sporozoite motility, plays an important role in the traversal of host cells during the preerythrocytic stage of Plasmodium species. Recently, it has been considered an alternative target when designing novel antimalarial vaccines against Plasmodium falciparum. However, the potential of Plasmodium vivax CelTOS as a vaccine target is yet to be explored. This study evaluated the naturally acquired immune response against a recombinant P. vivax CelTOS (PvCelTOS) (IgG and IgG subclass) in 528 individuals from Brazilian Amazon, as well as the screening of B-cell epitopes in silico and peptide assays to associate the breadth of antibody responses of those individuals with exposition and/or protection correlates. We show that PvCelTOS is naturally immunogenic in Amazon inhabitants with 94 individuals (17.8%) showing specific IgG antibodies against the recombinant protein. Among responders, the IgG reactivity indexes (RIs) presented a direct correlation with the number of previous malaria episodes (p = 0.003; r = 0.315) and inverse correlation with the time elapsed from the last malaria episode (p = 0.031; r = -0.258). Interestingly, high responders to PvCelTOS (RI > 2) presented higher number of previous malaria episodes, frequency of recent malaria episodes, and ratio of cytophilic/non-cytophilic antibodies than low responders (RI < 2) and non-responders (RI < 1). Moreover, a high prevalence of the cytophilic antibody IgG1 over all other IgG subclasses (p < 0.0001) was observed. B-cell epitope mapping revealed five immunogenic regions in PvCelTOS, but no associations between the specific IgG response to peptides and exposure/protection parameters were found. However, the epitope (PvCelTOSI136-E143) was validated as a main linear B-cell epitope, as 92% of IgG responders to PvCelTOS were also responders to this peptide sequence. This study describes for the first time the natural immunogenicity of PvCelTOS in Amazon individuals and identifies immunogenic regions in a full-length protein. The IgG magnitude was mainly composed of cytophilic antibodies (IgG1) and associated with recent malaria episodes. The data presented in this paper add further evidence to consider PvCelTOS as a vaccine candidate. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | University of Oxford. The Henry Wellcome Building for Molecular Physiology. The Jenner Institute. Nuffield Department of Medicine. Oxford, UK. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Grupo de Modelagem Computacional. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas. Laboratório de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Pesquisas da Amazônia. Laboratório de Leishmanioses e Doença de Chagas. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Pesquisas da Amazônia. Laboratório de Leishmanioses e Doença de Chagas. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | University of Oxford. The Henry Wellcome Building for Molecular Physiology. The Jenner Institute. Nuffield Department of Medicine. Oxford, UK. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | PvCelTOS | pt_BR |
| Subject | P. vivax | pt_BR |
| Subject | vaccines | pt_BR |
| Subject | epitope mapping | pt_BR |
| Subject | epitope prediction | pt_BR |
| Subject | malaria vaccines | pt_BR |
| Subject | malaria | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
