| Author | Lopes, Josiane Valadão | |
| Author | Michalsky, Érika Monteiro | |
| Author | Silva, Fabiana de Oliveira Lara | |
| Author | Lima, Ana Cristina Vianna Mariano da Rocha | |
| Author | Avelar, Daniel Moreira de | |
| Author | Costa, Ailton Antunes Junior da | |
| Author | Silva, João Carlos França | |
| Author | Silva, Shara Regina | |
| Author | Dias, Consuelo Latorre Fortes | |
| Author | Dias, Edelberto Santos | |
| Access date | 2018-06-26T12:44:34Z | |
| Available date | 2018-06-26T12:44:34Z | |
| Document date | 2017 | |
| Citation | LOPES, Josiane Valadão et al. Seroprevalence and molecular characterization of Leishmania in dogs from an endemic area of zoonotic visceral leishmaniasis in Brazil. International Journal of Veterinary Science and Medicine, v. 5, n. 1, p. 70-74, 2017 | pt_BR |
| ISSN | 2314-4599 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27098 | |
| Language | eng | pt_BR |
| Publisher | Elsevier B.V. | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | leishmaniose visceral canina | pt_BR |
| Subject in Portuguese | LnPCR | pt_BR |
| Title | Seroprevalence and molecular characterization of Leishmania in dogs from an endemic area of zoonotic visceral leishmaniasis in Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ijvsm.2017.02.002 | |
| Abstract | Current seroprevalence and circulating Leishmania species were evaluated in dogs domiciled in the municipality of Sabará, a small historic and touristic city in the Brazilian state of Minas Gerais. A total of 3926 dogs domiciled in seven different districts of Sabará were serologically tested for canine visceral leishmaniasis (CVL) by indirect enzyme-linked immunosorbent (ELISA) and immunofluorescence (IFA) assays, in a two-years census survey (2011–2012). The average positivity rate of canine infection was 3.4%. Three additional diagnostic tests – imprint/smear direct parasitological, molecular (LnPCR) and myeloculture – were performed in a random sample of fifty seropositive dogs composed of symptomatic (39) and asymptomatic (eleven) animals. LnPCR showed 100% of positivity for Leishmania DNA in, at least, one among four tissue samples tested (mesenteric lymph node, skin, spleen and bone marrow), independently of the clinical canine group. Higher and statistically equivalent positivity rates (98% and 96%) for Leishmania DNA were found in canine lymph node and spleen. Asymptomatic dogs showed expressive positivity rates in all three additional diagnostic techniques. Leishmania infantum was confirmed as the etiological agent of CVL in Sabará. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte. MG, Brazil | pt_BR |
| Subject | Canine visceral leishmaniasis | pt_BR |
| Subject | LnPCR | pt_BR |
| Subject | Myeloculture | pt_BR |
| Subject | Dogs | pt_BR |
| Subject | Leishmania infantum | pt_BR |
| Embargo date | 2023-01-01 | |
