Author | Lemos, Anderson José Gonzaga | |
Author | Balvedi, Renata Pereira Alves | |
Author | Rodovalho, Vinicius de Resende | |
Author | Resende, Laise Oliveira | |
Author | Castro, Ana Cristina Honorato | |
Author | Cuadros-Orellana, Sara | |
Author | Madurro, João Marcos | |
Author | Madurro, Ana Graci Brito | |
Access date | 2018-06-27T14:34:48Z | |
Available date | 2018-06-27T14:34:48Z | |
Document date | 2017 | |
Citation | LEMOS, Anderson José Gonzaga et al. Immunosensor assembled on polymeric nanostructures for clinical diagnosis of C-reactive protein. Microchemical Journal, v. 133, p. 572-576, 2017 | pt_BR |
ISSN | 0026-265X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27153 | |
Language | eng | pt_BR |
Publisher | Elsevier B.V | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | biosensores | pt_BR |
Subject in Portuguese | proteína C-reativa | pt_BR |
Subject in Portuguese | Antígeno-anticorpo de Doenças cardiovasculares | pt_BR |
Title | Immunosensor assembled on polymeric nanostructures for clinical diagnosis of C-reactive protein | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.microc.2017.04.026 | |
Abstract | This paper describes the development of an immunosensor for the c-reactive protein (CRP), based on both direct and indirect electrochemical detections. The anti-CRP probe was grafted to the platform sensitized with poly(3-aminothiophenol) and the interaction of the CRP target with the immunosensor was carried either directly or indirectly, using 4-aminoantipyrine (4-APP) as indicator. The immunosensor presents a linear response in the range from 75 ng•mL− 1 to 150 μg•mL− 1 and detection limit of 7.24 μg•mL− 1 (N = 3). The biosensor specificity, which was determined in the presence of non-specific target is also presented in this paper. Assays realized conducted with real samples indicate that the immunosensor discriminates the serum samples from healthy and sick patients with efficiency. In addition, through atomic force microscopy, it was possible to observe differences between the immunosensor surface in the absence or presence of the CRP target. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Faculdade de Engenharia Biomédica. Uberlândia, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Química. Uberlândia, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brasil | pt_BR |
Subject | Biosensors | pt_BR |
Subject | C-reactive protein | pt_BR |
Subject | Cardiovascular diseases Antigen-antibody | pt_BR |
Subject | recognition | pt_BR |
Subject | Poly(3-aminothiophenol) | pt_BR |
Embargo date | 2023-01-01 | |