| Author | Reis, Bruno Luiz Fonseca Schamber | |
| Author | Nardelli, Sheila | |
| Author | Silva, Carlos Gustavo Régis | |
| Author | Campos, Priscila Carneiro | |
| Author | Cerqueira, Paula Gonçalves | |
| Author | Lima, Sabrina Almeida | |
| Author | Franco, Glória Regina | |
| Author | Macedo, Andrea Mara | |
| Author | Pena, Sergio Danilo Junho | |
| Author | Cazaux, Christophe | |
| Author | Hoffmann, Jean-Sébastien | |
| Author | Motta, Maria Cristina Machado | |
| Author | Schenkman, Sergio | |
| Author | Teixeira, Santuza Maria Ribeiro | |
| Author | Machado, Carlos Renato | |
| Access date | 2018-06-29T16:24:58Z | |
| Available date | 2018-06-29T16:24:58Z | |
| Document date | 2012 | |
| Citation | REIS, B. L. F. S. et al. DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions. Molecular and Biochemical Parasitology, v. 183, p. 122– 131, 2012. | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27201 | |
| Description | Silva, Carlos Gustavo Régis. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Sponsorship | CNPq-Brazil (MCT/CNPq/MSSCTIE-DECIT 25/2006-Estudo de Doenc¸ as Negligenciadas), PRONEX
and FAPEMIG. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Reparo de DNA | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Estresse oxidativo | pt_BR |
| Subject in Portuguese | Oxoguanine | pt_BR |
| Subject in Portuguese | Kinetoplast | pt_BR |
| Title | DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.molbiopara.2012.02.007 | |
| Abstract | Specific DNA repair pathways from Trypanosoma cruzi are believed to protect genomic DNA and kinetoplast DNA (kDNA) from mutations. Particular pathways are supposed to operate in order to repair nucleotides oxidized by reactive oxygen species (ROS) during parasite infection, being 7,8-dihydro-8-oxoguanine (8oxoG) a frequent and highly mutagenic base alteration. If unrepaired, 8oxoG can lead to cytotoxic base transversions during DNA replication. In mammals, DNA polymerase beta (Polβ) is mainly involved in base excision repair (BER) of oxidative damage. However its biological role in T. cruzi is still unknown. We show, by immunofluorescence localization, that T. cruzi DNA polymerase beta (Tcpolβ) is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes. Nevertheless, this polymerase was detected inside the mitochondrial matrix of trypomastigote forms, which are not able to replicate in culture. Parasites over expressing Tcpolβ showed reduced levels of 8oxoG in kDNA and an increased survival after treatment with hydrogen peroxide when compared to control cells. However, this resistance was lost after treating Tcpolβ overexpressors with methoxiamine, a potent BER inhibitor. Curiously, a presumed DNA repair focus containing Tcpolβ was identified in the vicinity of kDNA of cultured wild type epimastigotes after treatment with hydrogen peroxide. Taken together our data suggest participation of Tcpolβ during kDNA replication and repair of oxidative DNA damage induced by genotoxic stress in this organelle. | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil / School of Medical Sciences. Superior Learning and Development Center. Campina Grande, PB, Brazil | pt_BR |
| Affilliation | Federal University of São Paulo. Microbiology, Immunology and Parasitology Department. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | University of Toulouse. Institute of Pharmacology and Structural Biology. Toulouse, France | pt_BR |
| Affilliation | University of Toulouse. Institute of Pharmacology and Structural Biology. Toulouse, France | pt_BR |
| Affilliation | Federal University of Rio de Janeiro. Institute of Biophysics Carlos Chagas Filho. Hertha Meyer Cellular Ultra structure Laboratory. Rio de Janeiro, RJ, Brazil | pt_BR |
| Affilliation | Federal University of São Paulo. Microbiology, Immunology and Parasitology Department. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | DNA repair | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Oxidative stress | pt_BR |
| Subject | Oxoguanine | pt_BR |
| Subject | Kinetoplast | pt_BR |
