| Author | Queiróz, Artur Trancoso Lopo de | |
| Author | Coutinho, Vinicius Ramos Hentique Maracajá | |
| Author | Jardim, Ana Carolina Gomes | |
| Author | Rahal, Paula | |
| Author | Mello, Isabel Maria Vicente Guedes de Carvalho | |
| Author | Matioli, Sergio Russo | |
| Access date | 2018-07-03T13:57:16Z | |
| Available date | 2018-07-03T13:57:16Z | |
| Document date | 2011 | |
| Citation | QUEIROZ, A. T. L. et al. Relation of pretreatment sequence diversity in NS5A region of HCV genotype 1 with immune response between pegylated-INF/ribavirin therapy outcomes. Journal of Viral Hepatitis, v. 18, p. 142–148, 2011. | pt_BR |
| ISSN | 1352-0504 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27230 | |
| Description | Queiróz, Artur Trancoso Lopo de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Sponsorship | CAPES | pt_BR |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Genótipo 1 | pt_BR |
| Subject in Portuguese | Vírus da hepatite C | pt_BR |
| Subject in Portuguese | Hepatite | pt_BR |
| Subject in Portuguese | Resposta imune | pt_BR |
| Subject in Portuguese | NS5A | pt_BR |
| Title | Relation of pretreatment sequence diversity in NS5A region of HCV genotype 1 with immune response between pegylated-INF/ribavirin therapy outcomes | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1111/j.1365-2893.2010.01294.x | |
| Abstract | Hepatitis C virus (HCV) infection frequently persists despite substantial virus-specific immune responses and the combination of pegylated interferon (INF)-α and ribavirin therapy. Major histocompatibility complex class I restricted CD8(+) T cells are responsible for the control of viraemia in HCV infection, and several studies suggest protection against viral infection associated with specific HLAs. The reason for low rates of sustained viral response (SVR) in HCV patients remains unknown. Escape mutations in response to cytotoxic T lymphocyte are widely described; however, its influence in the treatment outcome is ill understood. Here, we investigate the differences in CD8 epitopes frequencies from the Los Alamos database between groups of patients that showed distinct response to pegylated α-INF with ribavirin therapy and test evidence of natural selection on the virus in those who failed treatment, using five maximum likelihood evolutionary models from PAML package. The group of sustained virological responders showed three epitopes with frequencies higher than Non-responders group, all had statistical support, and we observed evidence of selection pressure in the last group. No escape mutation was observed. Interestingly, the epitope VLSDFKTWL was 100% conserved in SVR group. These results suggest that the response to treatment can be explained by the increase in immune pressure, induced by interferon therapy, and the presence of those epitopes may represent an important factor in determining the outcome of therapy. | pt_BR |
| Affilliation | São Paulo University. Biosciences Institute. Department of Genetics and Evolutive Biology. São Paulo, SP, Brasil | pt_BR |
| Affilliation | São Paulo University. Chemistry Institute. Biochemistry Department. São Paulo, SP, Brasil | pt_BR |
| Affilliation | São Paulo University. Instituto de Biociências, Letras e Ciências Exatas. Department of Biology. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Butantan Institute. Viral Immunology Laboratory. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Butantan Institute. Viral Immunology Laboratory. São Paulo, SP, Brasil | pt_BR |
| Affilliation | São Paulo University. Biosciences Institute. Department of Genetics and Evolutive Biology. São Paulo, SP, Brasil | pt_BR |
| Subject | Genotype 1 | pt_BR |
| Subject | Hepatitis C virus | pt_BR |
| Subject | Hepatitis | pt_BR |
| Subject | Immune response | pt_BR |
| Subject | NS5A | pt_BR |
