| Author | Bernardo, Marcela Piassi | |
| Author | Coelho, Luciana Fontes | |
| Author | Lima, Cristian J. Bolner de | |
| Author | Rodovalho, Cynara de Melo | |
| Author | Oliveira, Paola Monteiro de | |
| Author | Paula, Fabrício C. de | |
| Author | Contiero, Jonas | |
| Access date | 2018-07-03T16:30:17Z | |
| Available date | 2018-07-03T16:30:17Z | |
| Document date | 2013 | |
| Citation | BERNARDO, Marcela Piassi; et al. Isolation and characterization of bacterial producers of optically pure D(-) and L(+) lactic acid. African Journal of Microbiology Resaerch, v.7, n.21, p.2618-2628, May 2013. | pt_BR |
| ISSN | 1996-0808 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27242 | |
| Language | eng | pt_BR |
| Publisher | Academic Journals | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Lactic acid bacteria | pt_BR |
| Subject in Portuguese | Identificação | pt_BR |
| Subject in Portuguese | Caracterização | pt_BR |
| Subject in Portuguese | Optimização | pt_BR |
| Subject in Portuguese | Ácido Láctico | pt_BR |
| Subject in Portuguese | fonte de carbono | pt_BR |
| Title | Isolation and characterization of bacterial producers of optically pure D(-) and L(+) lactic acid | pt_BR |
| Type | Article | |
| DOI | 10.5897/AJMR2013.5388 | |
| Abstract | The aim of the present study was to isolate and characterize bacterial producers of optically pure D(-)
and L(+) lactic acid for use in the synthesis of polymers employed in the production of resistive and
biodegradable materials. Four hundred microorganisms were isolated. Of these strains, 20 had high
capacity for production of only one lactic acid isomer which was selected for characterization. D(-)
lactic acid producers were identified as Weissella paramesenteroides (25%), Leuconostoc
mesenteroides (6.25%), Leuconostoc lactis (6.25%) and Lactobacillus delbrueckii (6.25%). L(+) lactic
acid producers were identified as Lactobacillus casei (12.5%), Enterococcus sp. (18.75%), Lactobacillus
paracasei (6.25%), Streptococcus sp. (6.25%) and Lactobacillus sp. (12.5%). The most promising isolate
for L(+) lactic acid production was Ke8, in the presence of sucrose. Otherwise, Ke6 and Ke11 were the
best producers, by using glucose as the carbon source. The best microorganism for the production of
D(-) lactic acid was isolated from fermented milk (L. delbrueckii Y15C). However, the growth of this
microorganism is easily inhibited on media containing sugar concentration greater than 60 g/L. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Centro de Estudos de Insetos Sociais. Rio Claro, SP, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioquímica e Microbiologia. Rio Claro, SP, Brasil. | pt_BR |
| Subject | Lactic acid bacteria | pt_BR |
| Subject | dentification | pt_BR |
| Subject | characterization | pt_BR |
| Subject | carbon sources | pt_BR |
| Subject | lactic acid | pt_BR |
| Subject | optimization | pt_BR |
| e-ISSN | 1996-0808 | |
