Author | Bafica, Aline Michelle Barbosa | |
Author | Cardoso, Luciana Santos | |
Author | Oliveira, Sérgio Costa | |
Author | Loukas, Alex | |
Author | Góes, Alfredo | |
Author | Oliveira, Ricardo Riccio | |
Author | Carvalho Filho, Edgar Marcelino | |
Author | Araujo, Maria Ilma | |
Access date | 2018-07-03T18:39:25Z | |
Available date | 2018-07-03T18:39:25Z | |
Document date | 2012 | |
Citation | BAFICA, A. M. B. et al. Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients. Journal of Parasitology Research, Article ID 520308, 2012. | pt_BR |
ISSN | 2090-0023 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27254 | |
Description | Oliveira, Ricardo Riccio; Carvalho Filho, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Sponsorship | Brazilian National Research Council (CNPq) and by the NIH Grant no. NIH AI 088650. | pt_BR |
Language | eng | pt_BR |
Publisher | Hindawi Publishing Corporation | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Schistosoma mansoni | pt_BR |
Subject in Portuguese | Leishmaniose Tegumentar | pt_BR |
Subject in Portuguese | Células T | pt_BR |
Subject in Portuguese | Monócitos | pt_BR |
Subject in Portuguese | Leishmaniose cutânea | pt_BR |
Subject in Portuguese | Antígenos | pt_BR |
Title | Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients | pt_BR |
Type | Article | pt_BR |
DOI | 10.1155/2012/520308 | |
Abstract | High levels of proinflammatory cytokines such as IFN-γ and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that Schistosoma mansoni antigens downmodulate the in vitro cytokine response in CL. In the current study we evaluated whether S. mansoni antigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured with L. braziliensis antigen in the presence or absence of the S. mansoni antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14(+)CD16(++)) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14(++)CD16(-)) and intermediate (CD14(++)CD16(+)) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4(+) T cells and they also expanded the frequency of CD4(+)CD25(high)Foxp3(+) T cells. Taken together, we show that S. mansoni antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes. | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Instituto Nacional de Ciˆencia e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
Affilliation | James Cook University. School of Public Health and Tropical Medicine. Queensland Tropical Health Alliance. Cairns, Australia | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | Tegumentary leishmaniasis | pt_BR |
Subject | T-Cell | pt_BR |
Subject | Monocyte | pt_BR |
Subject | Cutaneous leishmaniasis | pt_BR |
Subject | Antigens | pt_BR |