Author | Melo, Stella Maria Barrouin | |
Author | Larangeira, Daniela Farias | |
Author | Santos, Silvana Ornelas | |
Author | Chagas Júnior, Adenizar Delgado das | |
Author | Paixão, Mariza S | |
Author | Aguiar, Paulo Henrique Palis | |
Author | dosSantos, Washington Luis Conrado | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Access date | 2011-07-01T19:16:02Z | |
Available date | 2011-07-01T19:16:02Z | |
Document date | 2006 | |
Citation | BARROUIN-MELO, S. M. et al. A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis. Veterinary Immunology and Immunopathology, v. 111, n. 3-4, p. 251-261, 2006. | pt_BR |
ISSN | 0165-2427 | |
URI | https://www.arca.fiocruz.br/handle/icict/2742 | |
Language | eng | pt_BR |
Rights | open access | en |
Subject in Portuguese | Baço | pt_BR |
Subject in Portuguese | Canino | pt_BR |
Subject in Portuguese | Biópsia Aspirativa com Agulha Fina | pt_BR |
Subject in Portuguese | Populações de Leucócitos | pt_BR |
Subject in Portuguese | Leishmaniose Visceral Canina | pt_BR |
Title | A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis | pt_BR |
Type | Article | pt_BR |
Abstract | A method for the evaluation of splenic cellularity using samples collected by fine-needle aspirative biopsy was standardized
in this work. The procedure includes erythrocyte lysing, preparation of cytospin films and staining by histochemical and
immunocytochemical techniques. The cellular profiles of spleen preparations were compared with those observed in peripheral
blood samples subjected to the same procedure. Two groups were compared, one consisting of 14 healthy uninfected and the
other of 15 polysymptomatic Leishmania chagasi/infantum-infected dogs, from an endemic area for visceral leishmaniosis. Cell
populations were identified by conventional hematoxilin–eosin and Wright’ stainings, and by immunocytochemistry using
monoclonal antibodies against canine CD45RA and CD45RB, phagocytes and a pan-leukocyte antigen. Larger neutrophil
(P < 0.0001) and monocyte/macrophage (P = 0.0036) relative counts and lower lymphocyte relative counts (P < 0.0001) were
found in the spleen, and not in the blood, of the animals with leishmaniosis than in those of the healthy animals. The proportions
of CD45RB+ cells were higher, and of CD45RA+ cells were lower, both in the spleen and in the blood of animals with leishmaniosis than in those of healthy dogs (P < 0.05). Additionally, hematoxilin–eosin-stained cytospins of spleen aspirates
from Leishmania-infected animals permitted the easy visualization of amastigote forms inside phagocytes, under light
microscopy. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Departamento de Patologia e Clínicas. Escola de Medicina Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Laboratório Central do Estado da Bahia, Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Departamento de Patologia e Clínicas. Escola de Medicina Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Subject | Spleen | pt_BR |
Subject | Canine | pt_BR |
Subject | Fine-needle Aspirative Biopsy | pt_BR |
Subject | Leukocyte Populations | pt_BR |
Subject | Canine Visceral Leishmaniosis | pt_BR |
DeCS | Baço | pt_BR |
DeCS | Biópsia por Agulha | pt_BR |
DeCS | Leucócitos | pt_BR |
DeCS | Leishmaniose Visceral | pt_BR |