| Author | Teixeira, Márcia Cristina Aquino | |
| Author | Oliveira, Geraldo Gileno de Sá | |
| Author | Silvany, Marco Antonio Araujo | |
| Author | Neves, Neuza Maria Alcântara | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Santos, Ricardo Ribeiro dos | |
| Author | Jerônimo, Selma Maria Bezerra | |
| Author | Costa, Carlos Henrique Nery | |
| Author | dosSantos, Washington Luis Conrado | |
| Author | Eichinger, Daniel | |
| Author | Pontes-de-Carvalho, Lain Carlos | |
| Access date | 2011-07-04T16:24:39Z | |
| Available date | 2011-07-04T16:24:39Z | |
| Document date | 2006 | |
| Citation | TEIXEIRA, M. C. A. et al. A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries. Biologicals, v.35, n.1, p.51-54, 2007. | pt_BR |
| ISSN | 1045-1056 | |
| URI | https://www.arca.fiocruz.br/handle/icict/2745 | |
| Language | eng | pt_BR |
| Rights | open access | en |
| Subject in Portuguese | Antígeno Recombinante | pt_BR |
| Subject in Portuguese | Biblioteca de cDNA | pt_BR |
| Subject in Portuguese | Sorodiagnóstico | pt_BR |
| Subject in Portuguese | Sensibilidade Imunoensaio | pt_BR |
| Subject in Portuguese | Leishmania Infantum | pt_BR |
| Subject in Portuguese | Leishmaniose Visceral | pt_BR |
| Title | A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.biologicals.2006.01.005 | |
| Abstract | Different individuals, when infected with the same parasite, rarely produce antibodies against the same set of antigens. Indeed, unless a particular
antigen happens to be recognized by antibodies in all individuals, the use of a single antigen in the serodiagnosis of parasitic diseases
leads, invariably, to false-negative results. A straightforward method for pin-pointing, in genetic libraries, the precise antigens that would increase
serodiagnostic assay sensitivities is presented. The method is based on the utilization of sera that produced false-negative results against previously
available antigens. Employing this false-negative serum-selection methodology for the identification of new Leishmania infantum
recombinant antigens (rAgs), the sensitivity of a dipstick assay for anti-Leishmania antibodies in a panel of sera from patients with visceral leishmaniasis
was increased from 83.3% to 98.1%, without affecting its specificity, by the inclusion of a fifth and a sixth L. infantum rAg. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Faculdade de Tecnologia e Ciências - FTC. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Faculdade de Tecnologia e Ciências - FTC. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Norte. Natal, RN, Brasil | pt_BR |
| Affilliation | Universidade Federal do Piauí. Teresina. Piauí, PI, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | New York University Medical Center. New York, NI, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Subject | Recombinant Antigen | pt_BR |
| Subject | cDNA Library | pt_BR |
| Subject | Serodiagnosis | pt_BR |
| Subject | Immunoassay Sensitivity | pt_BR |
| Subject | Leishmania Infantum | pt_BR |
| Subject | Visceral Leishmaniasis | pt_BR |
| DeCS | Biblioteca Gênica | pt_BR |
| DeCS | Testes Sorológicos | pt_BR |
| DeCS | Imunoensaio | pt_BR |
| DeCS | Leishmania Infantum | pt_BR |
| DeCS | Leishmaniose Visceral | pt_BR |
