Author | Caldas, Arlene de Jesus Mendes | |
Author | Favali, Cecilia Beatriz Fiuza | |
Author | Aquino, Dorlene Maria Cardoso de | |
Author | Vinhas, Vera | |
Author | Weyenbergh, Johan van | |
Author | Brodskyn, Claudia Ida | |
Author | Costa, Jackson Mauricio Lopes | |
Author | Barral Netto, Manoel | |
Author | Barral, Aldina Maria Prado | |
Access date | 2011-07-06T18:47:08Z | |
Available date | 2011-07-06T18:47:08Z | |
Document date | 2005 | |
Citation | CALDAS, A. et al. Balance of IL-10 and Interferon-γ plasma levels in human visceral leishmaniasis: implications in the pathogenesis. BMC Infectious Diseases, v.5, n.113, 2005. | pt_BR |
ISSN | 1471-2334 | |
URI | https://www.arca.fiocruz.br/handle/icict/2749 | |
Language | eng | pt_BR |
Rights | open access | en |
Subject in Portuguese | Leishmaniose Visceral Humana | pt_BR |
Subject in Portuguese | Imunorregulação | pt_BR |
Subject in Portuguese | Imunoprofilaxia | pt_BR |
Subject in Portuguese | Interferon-y | pt_BR |
Title | Balance of IL-10 and Interferon-γ plasma levels in human visceral leishmaniasis: implications in the pathogenesis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1471-2334-5-113 | |
Abstract | Background: Leishmaniasis remains a serious public health problem in several parts of the
developing world. Effective prophylactic measurements are hampered by imprecise comprehension
of different aspects of the disease, including its immunoregulation. A better comprehension of
immunoregulation in human VL may be useful both for designing and evaluating
immunoprophylaxis.
Methods: To explore immunoregulatory mechanisms, 20 visceral leishmaniasis (VL) patients were
evaluated during active disease and at different periods up to one year after treatment determining
their plasma cytokine levels, clinical parameters (palpable spleen and liver) and antibody levels.
Results: Elevated plasma levels of IFN-γ and of IL-12 p40 were observed during active disease,
significantly decreasing after treatment whereas in vitro Leishmania antigen-stimulated IFN-γ
production by PBMC exhibited an inverse pattern being low during disease and increasing steadily
thereafter. Absence of IFN-γ activity is a hallmark of VL. The main candidate for blunting IFN-γ
activity is IL-10, a cytokine highly elevated in plasma with sharp decrease after treatment. Activity
of IL-10 is inferred by high levels of anti-Leishmania specific IgG1 and IgG3. TGF-β had elevated
total, but not of active, levels lessening the likelihood of being the IFN-γ counterpart. Spleen or liver
size presented a steady decrease but return to normal values at only 120 days after treatment. Anti-
Leishmania IgG (total and subclasses) levels and DTH or Leishmania-stimulated lymphocyte
proliferation conversion to positive also present a slow decrease after treatment. IL-6 plasma levels
were elevated in only a few patients.
Conclusion: Taken together our results suggest that IFN-γ and IL-10 are the molecules most likely
involved in determining fate of disease. After treatment, there is a long delay before the immune
profile returns to normal what precludes using plasma cytokine levels as criteria of cure as simpler
clinical evaluations, as a palpable spleen or liver, can be used. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Departamento de Enfermagem. Universidade Federal do Maranhão. São Luís, MA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Departamento de Enfermagem. Universidade Federal do Maranhão. São Luís, MA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Instituto de Investigação em Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Instituto de Investigação em Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Instituto de Investigação em Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Instituto de Investigação em Imunologia. Salvador, BA, Brasil | pt_BR |
Subject | Human Visceral Leishmaniasis | pt_BR |
Subject | Immunoregulation | pt_BR |
Subject | Immunoprophylaxis | pt_BR |
Subject | Interferon-y | pt_BR |
DeCS | Leishmaniose Visceral | pt_BR |
DeCS | Interferon gama | pt_BR |