| Author | Souza, Milena Lázaro de | |
| Author | Matte, Christine | |
| Author | Lima, Jonilson Berlink | |
| Author | Duque, Guillermo Arango | |
| Author | Carvalho, Graziele Quintela de | |
| Author | Vivarini, Áislan de Carvalho | |
| Author | Pontes, Sara Moura | |
| Author | Figueira, Cláudio Pereira | |
| Author | Santos, Flávio Henrique de Jesus | |
| Author | Lopes, Ulisses Gazos | |
| Author | Farias, Leonardo Paiva | |
| Author | Santos, Théo de Araújo | |
| Author | Descoteaux, Albert | |
| Author | Borges, Valéria de Matos | |
| Access date | 2018-07-16T17:23:01Z | |
| Available date | 2018-07-16T17:23:01Z | |
| Document date | 2018 | |
| Citation | SOUZA, M. L. et al. Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay. Frontiers in Microbiology, v. 9, art 626, 2018. | pt_BR |
| ISSN | 1664-302X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27542 | |
| Sponsorship | Brazilian National Research Council (CNPq), (Grant number: 400135/2014-0 to VB),
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (Grant number: 04/2015 to VB), Science without Borders (SwB) (to VB and AD) and the Canadian Institutes of Health Research
(CIHR) (grant MOP-125990 to AD). VB and UG are sênior investigator funded by CNPq. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Lipofosfoglicano | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Direcionamento de genes | pt_BR |
| Subject in Portuguese | Gotículas lipídicas | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Title | Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3389/fmicb.2018.00626 | |
| Abstract | Lipophosphoglycan (LPG) is the major surface glycoconjugate of metacyclic Leishmania promastigotes and is associated with virulence in various species of this parasite. Here, we generated a LPG-deficient mutant of Leishmania infantum, the foremost etiologic agent of visceral leishmaniasis in Brazil. The L. infantum LPG-deficient mutant (Δlpg1) was obtained by homologous recombination and complemented via episomal expression of LPG1 (Δlpg1 + LPG1). Deletion of LPG1 had no observable effect on parasite morphology or on the presence of subcellular organelles, such as lipid droplets. While both wild-type and add-back parasites reached late phase in axenic cultures, the growth of Δlpg1 parasites was delayed. Additionally, the deletion of LPG1 impaired the outcome of infection in murine bone marrow-derived macrophages. Although no significant differences were observed in parasite load after 4 h of infection, survival of Δlpg1 parasites was significantly reduced at 72 h post-infection. Interestingly, L. infantum LPG-deficient mutants induced a strong NF-κB-dependent activation of the inducible nitric oxide synthase (iNOS) promoter compared to wild type and Δlpg1 + LPG1 parasites. In conclusion, the L. infantum Δlpg1 mutant constitutes a powerful tool to investigate the role(s) played by LPG in host cell-parasite interactions. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, Brasil | pt_BR |
| Affilliation | Institut National de la Recherche Scientifique. Institut Armand-Frappier. Laval, QC, Canada | pt_BR |
| Affilliation | Federal University of Western. Center of Biological Sciences and Health. Barreiras, BA, Brasil | pt_BR |
| Affilliation | Institut National de la Recherche Scientifique. Institut Armand-Frappier. Laval, QC, Canada | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, Brasil / Instituto Federal de Educação, Ciência e Tecnologia Baiano. Alagoinhas, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Carlos Chagas Filho Biophysics Institute. Center of Health Science. Laboratory of Molecular Parasitology. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Federal University of Bahia. Department of Legal Medicine. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Carlos Chagas Filho Biophysics Institute. Center of Health Science. Laboratory of Molecular Parasitology. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil | pt_BR |
| Affilliation | Federal University of Western. Center of Biological Sciences and Health. Barreiras, BA, Brasil | pt_BR |
| Affilliation | Institut National de la Recherche Scientifique. Institut Armand-Frappier. Laval, QC, Canada | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Department of Legal Medicine. Salvador, BA, Brasil | pt_BR |
| Subject | Lipophosphoglycan | pt_BR |
| Subject | Leishmania infantum | pt_BR |
| Subject | Gene targeting | pt_BR |
| Subject | Lipid droplets | pt_BR |
| Subject | Macrophage | pt_BR |
