| Author | Sodré, Cátia Lacerda | |
| Author | Chapeaurouge, Alexander D. | |
| Author | Kalume, Dário Eluan | |
| Author | Lima, Leila de Mendonça | |
| Author | Perales, Jonas | |
| Author | Fernandes, Octavio | |
| Access date | 2018-07-17T13:13:45Z | |
| Available date | 2018-07-17T13:13:45Z | |
| Document date | 2009 | |
| Citation | SODRÉ, Cátia Lacerda; et al. Proteomic map of Trypanosoma cruzi CL Brener: the reference strain of the genome project. Arch. Microbiol., v.191, p.177-184, 2009. | pt_BR |
| ISSN | 0302-8933 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27559 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Proteoma | pt_BR |
| Subject in Portuguese | Projeto Genoma | pt_BR |
| Title | Proteomic map of Trypanosoma cruzi CL Brener: the reference strain of the genome project | pt_BR |
| Type | Article | |
| DOI | 10.1007/s00203-008-0439-6 | |
| Abstract | In this work two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction of a map of soluble proteins from epimastigote form of Trypanosoma cruzi CL Brener. This strain is a hybrid organism derived from two genotypes, T. cruzi I and T. cruzi II and was chosen for genome sequencing. The two-dimensional gel electrophoresis showed that most of proteins focused at 4-7 pH range. The identification demonstrated that several proteins were in multiple isoforms, such as tubulin and heat shock proteins. Potential targets for development of chemotherapeutic agents like arginine kinase, an enzyme absent from mammalian tissues that is involved in the energy supply of the parasite, were also detected. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia Molecular de Doenças Infecciosas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxicologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia Molecular de Doenças Infecciosas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxicologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia Molecular de Doenças Infecciosas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Proteone | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Epimastigote | pt_BR |
| Subject | CL Brener strain | pt_BR |
| Subject | genome project | pt_BR |
| e-ISSN | 1432-072X | |
| Embargo date | 2030-01-01 | |
