| Author | Ghosh, Anil K. | |
| Author | Devenport, Martin | |
| Author | Jethwaney, Deepa | |
| Author | Kalume, Dario E. | |
| Author | Pandey, Akhilesh | |
| Author | Anderson, Vernon E. | |
| Author | Sultan, Ali A. | |
| Author | Kumar, Nirbhay | |
| Author | Jacobs-Lorena, Marcelo | |
| Access date | 2018-07-17T13:31:20Z | |
| Available date | 2018-07-17T13:31:20Z | |
| Document date | 2009 | |
| Citation | GHOSH, Anil K. et al. Malaria Parasite Invasion of the Mosquito Salivary Gland Requires Interaction between the Plasmodium TRAP and the Anopheles Saglin Proteins. PLoS Pathog., v.5, n.1, e1000265, 13p,, Jan. 2009. | pt_BR |
| ISSN | 1553-7366 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27561 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Malária | pt_BR |
| Subject in Portuguese | Invasão da Parasita | pt_BR |
| Subject in Portuguese | Glândula salivar | pt_BR |
| Subject in Portuguese | Mosquitos | pt_BR |
| Title | Malaria parasite invasion of the mosquito salivary gland requires interaction between the Plasmodium TRAP and the Anopheles saglin proteins | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.ppat.1000265 | |
| Abstract | SM1 is a twelve-amino-acid peptide that binds tightly to the Anopheles salivary gland and inhibits its invasion by Plasmodium sporozoites. By use of UV-crosslinking experiments between the peptide and its salivary gland target protein, we have identified the Anopheles salivary protein, saglin, as the receptor for SM1. Furthermore, by use of an anti-SM1 antibody, we have determined that the peptide is a mimotope of the Plasmodium sporozoite Thrombospondin Related Anonymous Protein (TRAP). TRAP binds to saglin with high specificity. Point mutations in TRAP's binding domain A abrogate binding, and binding is competed for by the SM1 peptide. Importantly, in vivo down-regulation of saglin expression results in strong inhibition of salivary gland invasion. Together, the results suggest that saglin/TRAP interaction is crucial for salivary gland invasion by Plasmodium sporozoites. | pt_BR |
| Affilliation | Johns Hopkins School of Public Health. Malaria Research Institute. Department of Molecular Microbiology and Immunology. Baltimore, Maryland, USA. | pt_BR |
| Affilliation | Johns Hopkins School of Public Health. Malaria Research Institute. Department of Molecular Microbiology and Immunology. Baltimore, Maryland, USA. | pt_BR |
| Affilliation | Harvard School of Public Health. Department of Immunology and Infectious Diseases. Boston, Massachussetts, USA. | pt_BR |
| Affilliation | Johns Hopkins School of Medicine. Departments of Biological Chemistry, Pathology, and Oncology. Baltimore, Maryland, USA / McKusick-Nathans Institute of Genetic Medicine. Baltimore, Maryland, USA . | pt_BR |
| Affilliation | Johns Hopkins School of Medicine. Departments of Biological Chemistry, Pathology, and Oncology. Baltimore, Maryland, USA / McKusick-Nathans Institute of Genetic Medicine. Baltimore, Maryland, USA . | pt_BR |
| Affilliation | Case Western Reserve University. Department of Biochemistry. Cleveland, Ohio, USA. | pt_BR |
| Affilliation | Harvard School of Public Health. Department of Immunology and Infectious Diseases. Boston, Massachussetts, USA. | pt_BR |
| Affilliation | Johns Hopkins School of Public Health. Malaria Research Institute. Department of Molecular Microbiology and Immunology. Baltimore, Maryland, USA. | pt_BR |
| Affilliation | Johns Hopkins School of Public Health. Malaria Research Institute. Department of Molecular Microbiology and Immunology. Baltimore, Maryland, USA. | pt_BR |
| Subject | Malaria parasite invasion | pt_BR |
| Subject | Mosquito salivary gland | pt_BR |
| Subject | Anopheles Saglin | pt_BR |
| Subject | Plasmodium TRAP | pt_BR |
| e-ISSN | 1553-7374 | |
