| Author | Vieira, J. M. B. D. | |
| Author | Seabra, S. H. | |
| Author | Vallim, D. C. | |
| Author | Américo, M.A. | |
| Author | Fracallanza, S. E. L. | |
| Author | Vommaro, R. C. | |
| Author | Domingues, R. M. C. P. | |
| Access date | 2018-07-17T16:49:16Z | |
| Available date | 2018-07-17T16:49:16Z | |
| Document date | 2009 | |
| Citation | VIEIRA, J. M. B. D. et al. Bacteroides fragilis induce necrosis on mice peritoneal macrophages: In vitro and in vivo assays. Biochemical and Biophysical Research Communications, v.387, p.627–632, 2009. | pt_BR |
| ISSN | 0006-291X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27575 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Bacteroides fragilis | pt_BR |
| Subject in Portuguese | Microscopia Eletrônica de Varredura | pt_BR |
| Subject in Portuguese | Apoptose | pt_BR |
| Subject in Portuguese | Necrose | pt_BR |
| Subject in Portuguese | Marcação In Situ das Extremidades Cortadas | pt_BR |
| Title | Bacteroides fragilis induce necrosis on mice peritoneal macrophages: In vitro and in vivo assays | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.bbrc.2009.05.124 | |
| Abstract | Bacteroides fragilis is an anaerobic bacteria component of human intestinal microbiota and agent of infections. In the host B. fragilis interacts with macrophages, which produces toxic radicals like NO. The interaction of activated mice peritoneal macrophages with four strains of B. fragilis was evaluated on this study. Previously was shown that such strains could cause metabolic and morphologic alterations related to macrophage death. In this work propidium iodide staining showed the strains inducing macrophage necrosis in that the labeling was evident. Besides nitroblue tetrazolium test showed that B. fragilis stimulates macrophage to produce oxygen radicals. In vivo assays performed in BalbC mice have results similar to those for in vitro tests as well as scanning electron microscopy, which showed the same surface pore-like structures observed in vitro before. The results revealed that B. fragilis strains studied lead to macrophage death by a process similar to necrosis. | pt_BR |
| Affilliation | Fundação Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Cultura de Células. Rio de Janeiro, RJ, Brasil / Fundação Centro Universitário Estadual da Zona Oeste. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Cultura de Células. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Bacteriologia Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Bacteriologia Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Bacteria/macrophage interactions | pt_BR |
| Subject | NO | pt_BR |
| Subject | TUNEL assay | pt_BR |
| Subject | PI staining | pt_BR |
| Subject | In vivo model | pt_BR |
| Subject | Apoptosis | pt_BR |
| Subject | Necrosis | pt_BR |
| Subject | Scanning electron microscopy | pt_BR |
| e-ISSN | 1090-2104 | |
| Embargo date | 2030-01-01 | |
