| Author | Campos, Fernanda Fraga | |
| Author | Ramos, Jonas Pereira | |
| Author | Oliveira, Djalma Menezes de | |
| Author | Alves, Tania Maria de Almeida | |
| Author | Fagundes, Elaine Maria de Souza | |
| Author | Zani, Carlos Leomar | |
| Author | Sampaio, Fabio Coelho | |
| Author | Converti, Attilio | |
| Author | Cota, Betania Barros | |
| Access date | 2018-07-19T17:45:57Z | |
| Available date | 2018-07-19T17:45:57Z | |
| Document date | 2017 | |
| Citation | CAMPOS, Fernanda Fraga et al. In vitro leishmanicidal, antibacterial and antitumour potential of anhydrocochlioquinone A obtained from the fungus Cochliobolus sp. J Biosci Vol. 42, No. 4, p. 657–664, 2017 | pt_BR |
| ISSN | 0250-5991 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27603 | |
| Language | eng | pt_BR |
| Publisher | Springer | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Anidrococlioquinona | pt_BR |
| Subject in Portuguese | Cochliobolus sp. | pt_BR |
| Subject in Portuguese | leishmanicida | pt_BR |
| Subject in Portuguese | antimicrobiano | pt_BR |
| Subject in Portuguese | células tumorais | pt_BR |
| Title | In vitro leishmanicidal, antibacterial and antitumour potential of anhydrocochlioquinone A obtained from the fungus Cochliobolus sp | pt_BR |
| Type | Article | |
| DOI | 10.1007/s12038-017-9718-1 | |
| Abstract | The bioassay-guided fractionation of the ethyl acetate extract of the fungus Cochliobolus sp. highlighted leishmanicidal activity and allowed for anhydrocochlioquinone A (ANDC-A) isolation. MS, 1D and 2D NMR spectra of this compound were in agreement with those published in the literature. ANDC-A exhibited leishmanicidal activity with EC50 value of 22.4 lg/mL (44 lM) and, when submitted to the microdilution assay against Gram-positive and Gram-negative bacteria, showed a minimal inhibitory concentration against Staphylococcus aureus ATCC 25295 of 128 lg/mL (248.7 lM). It was also active against five human cancer cell lines, showing IC50 values from 5.4 to 20.3 lM. ANDC-A demonstrated a differential selectivity for HL-60 (SI 5.5) and THP-1 (SI 4.3) cell lines in comparison with Vero cells and was more selective than cisplatin and doxorubicin against MCF-7 cell line in comparison with human peripheral blood mononuclear cells. ANDC-A was able to eradicate clonogenic tumour cells at concentrations of 20 and 50 lM and induced apoptosis in all tumour cell lines at 20 lM. These results suggest that ANDC-A might be used as a biochemical tool in the study of tumour cells biochemistry as well as an anticancer agent with durable effects on tumours. | pt_BR |
| Affilliation | Universidade Federal do Vale do Jequitinhonha e Mucuri. Faculdade de Medicina. Diamantina, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofisica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Estadual da Bahia. Departamento de Quimica. Jequie, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofisica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal do Vale do Jequitinhonha e Mucuri. Faculdade de Farmacia. Diamantina, MG, Brazil | pt_BR |
| Affilliation | Department of Civil, Chemical and Environmental Engineering. University of Genova. Genova, Italy | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Anhydrocochlioquinone | pt_BR |
| Subject | Cochliobolus sp. | pt_BR |
| Subject | leishmanicidal | pt_BR |
| Subject | antimicrobial | pt_BR |
| Subject | tumour cells | pt_BR |
| Embargo date | 2025-01-01 | |
