Author | Carvalho, Gustavo Mayr de Lima | |
Author | Brazil, Reginaldo Peçanha | |
Author | Rego, Felipe Dutra | |
Author | Zenóbio, Ana Paula Lusardo de Almeida | |
Author | Andrade Filho, José Dilermando | |
Access date | 2018-07-25T17:08:34Z | |
Available date | 2018-07-25T17:08:34Z | |
Document date | 2017 | |
Citation | CARVALHO, Gustavo Mayr de Lima et al. Molecular Detection of Leishmania DNA in Wild-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) From a Cave in the State of Minas Gerais, Brazil. Journal of Medical Entomology, v. 54, n. 1, p. 196-203, 2017. | pt_BR |
ISSN | 0022-2585 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27652 | |
Language | eng | pt_BR |
Publisher | Entomological Society of America | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Mosca de areia | pt_BR |
Subject in Portuguese | Caverna | pt_BR |
Subject in Portuguese | Detecção molecular | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Title | Molecular Detection of Leishmania DNA in Wild-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) From a Cave in the State of Minas Gerais, Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1093/jme/tjw137 | |
Abstract | Leishmania spp. are distributed throughout the world, and different species are associated with varying degrees of disease severity. In Brazil, Leishmaniatransmission involves several species of phlebotomine sand flies that are closely associated with different parasites and reservoirs, and thereby giving rise to different transmission cycles. Infection occurs during the bloodmeals of sand flies obtained from a variety of wild and domestic animals, and sometimes from humans. The present study focused on detection of Leishmania DNA in phlebotomine sand flies from a cave in the state of Minas Gerais. Detection of Leishmania in female sand flies was performed with ITS1 PCR-RFLP (internal transcribed spacer 1) using HaeIII enzyme and genetic sequencing for SSUrRNA target. The survey of Leishmania DNA was carried out on 232 pools and the parasite DNA was detected in four: one pool of Lutzomyia cavernicola (Costa Lima, 1932), infected with Le. infantum (ITS1 PCR-RFLP), two pools of Evandromyia sallesi (Galvão & Coutinho, 1939), both infected with Leishmania braziliensis complex (SSUrRNA genetic sequencing analysis), and one pool of Sciopemyia sordellii (Shannon & Del Ponte, 1927), infected with subgenus Leishmania (SSUrRNA genetic sequencing analysis). The present study identified the species for Leishmania DNA detected in four pools of sand flies, all of which were captured inside the cave. These results represent the first molecular detection of Lu. cavernicola with Le. infantum DNA, Sc. sordellii with subgenus Leishmania DNA, and Ev. sallesi with Leishmania braziliensis complex DNA. The infection rate in females captured for this study was 0.17%. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de doenças parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Sand fly | pt_BR |
Subject | Cave | pt_BR |
Subject | Molecular detection | pt_BR |
Subject | Leishmania | pt_BR |
Embargo date | 2100-01-01 | |