| Author | Araujo, Natalia M. | |
| Author | Vianna, Carlos O. A. | |
| Author | Moraes, Marcia T. B. | |
| Author | Gomes, Selma A. | |
| Access date | 2018-07-30T17:44:12Z | |
| Available date | 2018-07-30T17:44:12Z | |
| Document date | 2009 | |
| Citation | ARAUJO, Natalia M. et al. Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection. The Brazilian Journal of Infectious Diseases, v. 13, n. 4, p. 266-271, 2009. | pt_BR |
| ISSN | 1413-8670 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27720 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Virus da Hepatite B | pt_BR |
| Subject in Portuguese | Antígeno de superfície | pt_BR |
| Subject in Portuguese | Transfecção | pt_BR |
| Subject in Portuguese | Variações de aminoácidos | pt_BR |
| Title | Expression of Hepatitis B Virus Surface Antigen (HBsAg) from Genotypes A, D and F and Influence of Amino Acid Variations Related or Not to Genotypes on HBsAg Detection | pt_BR |
| Type | Article | |
| DOI | 10.1590/S1413-86702009000400005 | pt_BR |
| Abstract | The impact of hepatitis B virus (HBV) genotypes on the sensitivity of surface antigen (HBsAg) detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg detection values of consensus forms from genotypes D and F were, respectively, 37% and 30% lower than those obtained by genotype A. However, the presence of two single variations, T143M in genotype A, and T125M in genotype D, produced a decrease of 44% and an increase of 34%, respectively, on HBsAg mean values in comparison with their consensus forms. In conclusion, HBsAg detection levels varied among HBV genotypes. However, unique amino acid substitutions not linked to
genotypes, such as T125M and T143M described here, should have more implications in HBV immunological diagnostics than the set of variations characteristic of each HBV genotype. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Vice Direção de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Hepatitis B virus | pt_BR |
| Subject | Surface antigen | pt_BR |
| Subject | Amino acid variations | pt_BR |
| Subject | Transfection | pt_BR |
| e-ISSN | 1678-4391 | |
