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AutorNascimento, Eduardo J. M.
AutorGeorge, James K.
AutorVelasco, Melissa
AutorBonaparte, Matthew I.
AutorZheng, Lingyi
AutorDiazGranados, Carlos A.
AutorMarques, Ernesto T. A.
AutorHuleatt, James W.
Fecha de acceso2018-08-01T14:43:19Z
Fecha de disponibilización2018-08-01T14:43:19Z
Fecha de publicación2018
ReferenciaNASCIMENTO, Eduardo J. M. et al. Development of an Anti-Dengue NS1 IgG ELISA to Evaluate Exposure to Dengue Virus. Journal of Virological Methods, v. 257, p. 48–57, 2018.pt_BR
ISSN1879-0984pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/27762
PromociónSanofi Pasteurpt_BR
Idiomaengpt_BR
EditorElsevierpt_BR
Derechos de autorrestricted accesspt_BR
Palabras clave en PortuguésEnsaio de imunoabsorção enzimáticapt_BR
Palabras clave en PortuguésFlaviviruspt_BR
Palabras clave en PortuguésEnsaio de neutralizaçãopt_BR
Palabras clave en PortuguésProteína Não-Estrutural 1pt_BR
Palabras clave en PortuguésVírus da denguept_BR
Palabras clave en PortuguésIgGpt_BR
Palabras clave en PortuguésSoroprevalênciapt_BR
TítuloDevelopment of an anti-dengue NS1 IgG ELISA to evaluate exposure to dengue viruspt_BR
Tipo del documentoArticle
DOI10.1016/j.jviromet.2018.03.007
Resumen en InglésDengue virus infection elicits immune responses to multiple viral antigens including antibodies to dengue non-structural protein 1 (NS1) which are rapidly induced and detected within days of infection. The recombinant, live, attenuated, tetravalent dengue vaccine (CYD-TDV; Sanofi Pasteur) uses the yellow fever vaccine virus as a back-bone but expresses dengue virus pre-membrane and envelop proteins. Since CYD-TDV does not express dengue NS1, we evaluated the utility of dengue NS1-specific IgG antibodies as biomarkers of dengue exposure in CYD-TDV recipients and controls. We optimized and evaluated a quantitative anti-dengue NS1 IgG enzyme-linked immunosorbent assay (ELISA). Parameters assessed included: accuracy, dilutability/linearity, precision, limit of quantitation and specificity. The assay specificity was further evaluated using Japanese Encephalitis virus, West Nile virus, Yellow Fever virus or Zika virus positive sera samples collected following confirmed infection or vaccination. Receiver-operating-characteristics (ROC) curves as well as sensitivity and specificity for discriminating previous dengue exposure were assessed using 1250 reference samples. Overall, the anti-dengue NS1 IgG ELISA was able to discriminate previous dengue exposure from non-exposure before vaccination with CYD-TDV (ROC area under the curve > 0.9). Assessment of paired samples from 2511 vaccinated participants showed high overall agreement (93%) between pre-vaccination and post-vaccination dengue serostatus classification based on the anti-dengue NS1 IgG ELISA. However, misclassification of dengue serostatus was observed after vaccination likely due to a combination of asymptomatic dengue infections, assay variability and a modest effect of CYD-TDV on the anti-dengue NS1 IgG ELISA readout.pt_BR
AfiliaciónSanofi Pasteur. Discovery Drive. Swiftwater, PA, USA.pt_BR
AfiliaciónSanofi Pasteur. Discovery Drive. Swiftwater, PA, USA.pt_BR
AfiliaciónSanofi Pasteur. Discovery Drive. Swiftwater, PA, USA.pt_BR
AfiliaciónSanofi Pasteur. Discovery Drive. Swiftwater, PA, USA.pt_BR
AfiliaciónSanofi Pasteur. Discovery Drive. Swiftwater, PA, USA.pt_BR
AfiliaciónSanofi Pasteur. Discovery Drive. Swiftwater, PA, USA.pt_BR
AfiliaciónUniversity of Pittsburgh. Graduate School of Public Health and Center for Vaccine Research. Biomedical Science Tower 3. Pittsburgh, PA, USA / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.pt_BR
Palavras clave en InglêsEnzyme-Linked immunosorbent assaypt_BR
Palavras clave en InglêsFlaviviruspt_BR
Palavras clave en InglêsNeutralization assaypt_BR
Palavras clave en InglêsNon-Structural protein 1pt_BR
Palavras clave en InglêsDengue viruspt_BR
Palavras clave en InglêsIgGpt_BR
Palavras clave en InglêsSeroprevalencept_BR
Fecha de embargo2050-01-01


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