| Author | Faria, Tatiany J. de | |
| Author | Roman, Mariane | |
| Author | Souza, Nicole M. de | |
| Author | De Vecchi, Rodrigo | |
| Author | Assis, João Vitor de | |
| Author | Santos, Ana Lúcia Gomes dos | |
| Author | Bechtold, Ivan H. | |
| Author | Winter, Nathalie | |
| Author | Soares, Maurilio José | |
| Author | Silva, Luciano Paulino | |
| Author | Almeida, Mauro Vieira de | |
| Author | Báfica, André | |
| Access date | 2018-08-02T14:09:04Z | |
| Available date | 2018-08-02T14:09:04Z | |
| Document date | 2012 | |
| Citation | FARIA, Tatiany J. de et al. An Isoniazid Analogue Promotes Mycobacterium tuberculosis-Nanoparticle Interactions and Enhances Bacterial Killing by Macrophages. Antimicrobial Agents and Chemotherapy, v. 56, n. 5, p. 2259-2267, 2012. | pt_BR |
| ISSN | 0066-4804 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/27792 | |
| Language | por | pt_BR |
| Publisher | American Society For Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | JVA | pt_BR |
| Title | An isoniazid analogue promotes Mycobacterium tuberculosis-nanoparticle interactions and enhances bacterial killing by macrophages | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/AAC.05993-11 | |
| Abstract | Nanoenabled drug delivery systems against tuberculosis (TB) are thought to control pathogen replication by targeting antibiotics to infected tissues and phagocytes. However, whether nanoparticle (NP)-based carriers directly interact with Mycobacterium tuberculosis and how such drug delivery systems induce intracellular bacterial killing by macrophages is not defined. In the present study, we demonstrated that a highly hydrophobic citral-derived isoniazid analogue, termed JVA, significantly increases nanoencapsulation and inhibits M. tuberculosis growth by enhancing intracellular drug bioavailability. Importantly, confocal and atomic force microscopy analyses revealed that JVA-NPs associate with both intracellular M. tuberculosis and cell-free bacteria, indicating that NPs directly interact with the bacterium. Taken together, these data reveal a nanotechnology-based strategy that promotes antibiotic targeting into replicating extra- and intracellular mycobacteria, which could actively enhance chemotherapy during active TB. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Imunobiologia. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Imunobiologia. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Imunobiologia. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Imunobiologia. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Química. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Ciências Farmacêuticas. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Física. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | INRA UR1282. Infectiologie Animale et Santé Publique. Nouzilly, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | EMBRAPA Recursos Genéticos e Biotecnologia. Laboratório de Espectrometria de Massas, Brasília, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Química. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Imunobiologia. Florianópolis, SC, Brasil. | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | Isoniazid | pt_BR |
| Subject | Anti-Bacterial Agents | pt_BR |
| Subject | Drug Therapy | pt_BR |
| Subject in Spanish | Tratamiento Farmacológico | pt_BR |
| DeCS | Tuberculose | pt_BR |
| DeCS | Isoniazida | pt_BR |
| DeCS | Antibacterianos | pt_BR |
| DeCS | Tratamento Farmacológico | pt_BR |
| e-ISSN | 1098-6596 | |
