| Author | Pinheiro Junior, Nathanael F | |
| Author | Hermida, Micely D' El Rei | |
| Author | Macedo, Mariana P | |
| Author | Mengele Junior, José Orivaldo | |
| Author | Bafica, Andre Luiz Barbosa | |
| Author | dosSantos, Washington Luis Conrado | |
| Access date | 2011-08-11T15:46:56Z | |
| Available date | 2011-08-11T15:46:56Z | |
| Document date | 2006 | |
| Citation | PINHEIRO JUNIOR, N. F. et al. Leishmania infection impairs beta1-integrin function and chemokine receptor expression in mononuclear phagocytes. Infection and Immunity, v.74, n.7, p.3912–3921, jul. 2006. | pt_BR |
| ISSN | 0019-9567 | |
| URI | https://www.arca.fiocruz.br/handle/icict/2795 | |
| Description | Agradecemos a Lain C. Pontes-de-Carvalho, a Claudia Ida Brodskyn (ambos da FIOCRUZ, Centro de Pesquisa Gonçalo Moniz), e Antonio Rothfuchs (NIH / NIAID) pela revisão cuidadosa e as sugestões para o manuscrito. | pt_BR |
| Sponsorship | Este estudo foi financiado pela Fundação de Apoio à Pesquisa do Estado da Bahia (FAPESB) e pelo Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | pt_BR |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | en |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Quimiocinas | pt_BR |
| Title | Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/IAI.02103-05 | |
| Abstract | Leishmania spp. are intracellular parasites that cause lesions in the skin, mucosa, and viscera. We have previously shown that Leishmania infection reduces mononuclear phagocyte adhesion to inflamed connective
tissue. In this study, we examined the role of adhesion molecules and chemokines in this process. Infection rate (r 0.826, P 0.003) and parasite burden (r 0.917, P 0.028) negatively correlated to mouse phagocyte adhesion. The decrease (58.7 to 75.0% inhibition, P 0.005) in phagocyte adhesion
to connective tissue, induced by Leishmania, occurred as early as 2 h after infection and was maintained for at least 24 h. Interestingly, impairment of cell adhesion was sustained by phagocyte infection, since it was not observed following phagocytosis of killed parasites (cell adhesion varied from 15.2% below to 24.0% above control levels, P > 0.05). In addition, Leishmania infection diminished cell adhesion to fibronectin (54.1 to 96.2%, P < 0.01), collagen (15.7 to 83.7%, P < 0.05), and laminin (59.1 to 82.2%, P <
0.05). The CD11bhi subpopulation was highly infected (49.6 to 97.3%). Calcium and Mg2 replacement by Mn2 , a treatment that is known to induce integrins to a high state of affinity for their receptors, reverted
the inhibition in adhesion caused by Leishmania. This reversion was completely blocked by anti-VLA4 antibodies. Furthermore, expression of CCR4 and CCR5, two chemokine receptors implicated in cell adhesion, was found to be downregulated 16 h after infection (2.8 to 4.1 times and 1.9 to 2.8 times, respectively). Together, these results suggest that mechanisms regulating integrin function are implicated in the change of macrophage adhesion in leishmaniasis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / NIAID, National Institutes of Health. Laboratory of Parasitic Diseases. Immunobiology Section. Bethesda, Maryland, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Infection | pt_BR |
| Subject | Chemokines | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Antígenos CD29 | pt_BR |
| DeCS | Adesão Celular | pt_BR |
| DeCS | Leishmania braziliensis | pt_BR |
| DeCS | Leishmaniose Cutânea | pt_BR |
| DeCS | Macrófagos Peritoneais | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Camundongos Endogâmicos BALB C | pt_BR |
| DeCS | Receptores de Quimiocinas | pt_BR |
