| Author | Adesse, Daniel | |
| Author | Iacobas, Dumitru A. | |
| Author | Iacobas, Sanda | |
| Author | Garzoni, Luciana R. | |
| Author | Meirelles, Maria de Nazareth | |
| Author | Tanowitz, Herbert B. | |
| Author | Spray, David C. | |
| Access date | 2018-08-08T12:36:37Z | |
| Available date | 2018-08-08T12:36:37Z | |
| Document date | 2010 | |
| Citation | ADESSE, Daniel; et al. Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma Cruzi. Am. J. Trop. Med. Hyg., v.82, n.5, p.846–854, 2010. | pt_BR |
| ISSN | 0002-9637 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28000 | |
| Language | eng | pt_BR |
| Publisher | The American Society of Tropical Medicine and Hygiene | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma Cruzi | pt_BR |
| Subject in Portuguese | Assinaturas transcriptômicas | pt_BR |
| Subject in Portuguese | Linhagem mioblástica | pt_BR |
| Subject in Portuguese | Cepas | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi | pt_BR |
| Type | Article | |
| DOI | 10.4269/ajtmh.2010.09-0399 | |
| Abstract | We examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L(6)E(9) myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Department of Neuroscience. Bronx, NY, USA. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Department of Neuroscience. Bronx, NY, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Department of Pathology. Bronx, NY, USA. | pt_BR |
| Affilliation | Albert Einstein College of Medicine. Department of Neuroscience. Bronx, NY, USA. | pt_BR |
| Subject | Trypanosoma Cruzi | pt_BR |
| Subject | Transcriptomic Signatures | pt_BR |
| Subject | Myoblast Cell Line | pt_BR |
| Subject | Four Distinct Strains | pt_BR |
| Subject | Infection | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1476-1645 | |
