Mostrar el registro sencillo del ítem

AutorFrança, Luciana Souza de Aragão
AutorRocha, Viviane Costa Junqueira
AutorAndrade, André Cronemberger
AutorCosta, F H B
AutorVasconcelos, José Fernandes
AutorAthanazio, Daniel Abensur
AutorSilva, Daniela Nascimento
AutorSantos, Emanuelle de Souza
AutorMeira, Cássio Santana
AutorAraújo, Cintia Figueiredo de
AutorCerqueira, Jéssica Vieira
AutorCardillo, Fabíola
AutorNeves, Neuza Maria Alcântara
AutorSoares, Milena Botelho Pereira
AutorPontes-de-Carvalho, Lain Carlos
Fecha de acceso2018-08-10T13:45:55Z
Fecha de disponibilización2018-08-10T13:45:55Z
Fecha de publicación2018
ReferenciaFRANÇA, L. S. A. et al. Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation. Allergy Asthma and Immunology Research, v. 10, n. 4, p. 406-419, 2018.pt_BR
ISSN2092-7355pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/28055
PromociónPrograma de Excelência em Pesquisa (PROEP-CNPq).pt_BR
Idiomaengpt_BR
EditorThe Korean Academy of Asthma Allergy and Clinical Immunologypt_BR
Derechos de autoropen accesspt_BR
Palabras clave en PortuguésCélulas dendríticaspt_BR
Palabras clave en PortuguésAsmapt_BR
Palabras clave en PortuguésAlérgenospt_BR
Palabras clave en PortuguésÁcaros da poeira domésticapt_BR
Palabras clave en PortuguésImunoterapiapt_BR
Palabras clave en PortuguésIndução de tolerânciapt_BR
TítuloTolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammationpt_BR
Tipo del documentoArticlept_BR
DOI10.4168/aair.2018.10.4.406
Resumen en InglésThe use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte- macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme- linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónUniversidade de São Paulo. Departamento de Medicina. São Paulo, SP, Brasilpt_BR
AfiliaciónUniversity of Texas Health Science Center. Department of Diagnostics and Biomedical Sciences. Houston, USApt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónUniversidade Federal da Bahia. Hospital Universitário Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasilpt_BR
Palavras clave en InglêsDendritic cellspt_BR
Palavras clave en InglêsAsthmapt_BR
Palavras clave en InglêsAllergenspt_BR
Palavras clave en InglêsHouse dust mitespt_BR
Palavras clave en InglêsImmunotherapypt_BR
Palavras clave en InglêsTolerance inductionpt_BR
xmlui.metadata.dc.subject.ods11 Cidades e comunidades sustentáveis


Ficheros en el ítem

application/pdf
Nombre:
Aragão- França LS Tolerogenic ...
Tamaño:
1.833Mb
Formato:
application/
Ver/Abrir

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem