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AutorGonçalves, Gabrielle Viana Martins
AutorSilva, Daniela Nascimento
AutorCarvalho, Rejane Hughes
AutorSouza, Bruno Solano de Freitas
AutorSilva, Kátia Nunes da
AutorVasconcelos, Juliana Fraga
AutorParedes, Bruno Diaz
AutorNonaka, Carolina Kymie Vasques
AutorSantos, Ricardo Ribeiro dos
AutorSoares, Milena Botelho Pereira
Fecha de acceso2018-08-14T14:05:27Z
Fecha de disponibilización2018-08-14T14:05:27Z
Fecha de publicación2018
ReferenciaGONÇALVES, G. V. M. et al. Generation and characterization of transgenic mouse mesenchymal stem cell lines expressing hIGF-1 or hG-CSF. Cytotechnology, v. 70, p. 577–591, 2018.pt_BR
ISSN0920-9069pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/28128
PromociónThe National Council for Scientific and Technological Development (CNPq), The Foundation of Support for Research of the State of Bahia (FAPESB), and Funding Authority for Studies and Projects (FINEP).pt_BR
Idiomaengpt_BR
EditorSpringer Verlagpt_BR
Derechos de autoropen accesspt_BR
Palabras clave en PortuguésCélulas-tronco mesenquimaispt_BR
Palabras clave en PortuguésFatores de crescimentopt_BR
Palabras clave en PortuguésG-CSFpt_BR
Palabras clave en PortuguésIGF-1pt_BR
TítuloGeneration and characterization of transgenic mouse mesenchymal stem cell lines expressing hIGF-1 or hG-CSFpt_BR
Tipo del documentoArticlept_BR
DOI10.1007/s10616-017-0131-2
Resumen en InglésMesenchymal stem cells (MSC) are promising tools in the fields of cell therapy and regenerative medicine. In addition to their differentiation potential, MSC have the ability to secrete bioactive molecules that stimulate tissue regeneration. Thus, the overexpression of cytokines and growth factors may enhance the therapeutic effects of MSC. Here we generated and characterized mouse bone marrow MSC lines overexpressing hG-CSF or hIGF-1. MSC lines overexpressing hG-CSF or hIGF-1 were generated through lentiviral vector mediated gene transfer. The expression of hG-CSF or hIGF-1 genes in the clones produced was quantified by qRT-PCR, and the proteins were detected in the cell supernatants by ELISA. The cell lines displayed cell surface markers and differentiation potential into adipocytes, osteocytes and chondrocytes similar to the control MSC cell lines, indicating the conservation of their phenotype even after genetic modification. IGF-1 and G-CSF transgenic cells maintained immunosuppressive activity. Finally, we performed a comparative gene expression analysis by qRT-PCR array in the cell lines expressing hIGF-1 and hG-CSF when compared to the control cells. Our results demonstrate that the cell lines generated may be useful tools for cell therapy and are suitable for testing in disease models.pt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazilpt_BR
AfiliaciónHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazilpt_BR
Palavras clave en InglêsMesenchymal stem cellspt_BR
Palavras clave en InglêsGrowth factorspt_BR
Palavras clave en InglêsG-CSFpt_BR
Palavras clave en InglêsIGF-1pt_BR


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