| Author | Tuyama, Mary | |
| Author | Boente, Renata F. | |
| Author | Rebelo, Maria C. | |
| Author | Igreja, Ricardo P. | |
| Author | Barroso, David E. | |
| Access date | 2018-08-16T12:23:27Z | |
| Available date | 2018-08-16T12:23:27Z | |
| Document date | 2008 | |
| Citation | TUYAMA, Mary; et al. The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases. Mem Inst Oswaldo Cruz, vol.103, n.2, p.138-142, Mar. 2008. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28170 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Líquido Cefalorraquidiano | pt_BR |
| Subject in Portuguese | Meningite | pt_BR |
| Subject in Portuguese | Streptococcus pneumoniae | pt_BR |
| Subject in Portuguese | Neisseria meningitidis | pt_BR |
| Subject in Portuguese | Haemophilus influenzae | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Title | The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases | pt_BR |
| Type | Article | |
| Abstract | Most patients with acute suppurative meningitis are otherwise healthy individuals with regard to immune mechanisms against invasive bacterial disease. This medical emergency is among the most dramatic and potentially ravaging diseases that affect humans, particularly young children. The illness often strikes suddenly, and can either result in death or leave the survivors with significant neurological dysfunctions. The demonstration of a bacterial aetiology is necessary for decisions regarding treatment and prophylaxis. Conventional bacteriological methods frequently fail to identify an agent, as a result of administration of antibiotics or delayed lumbar punctures. We investigated the major aetiologic sources of unspecified bacterial meningitis cases (G00.9, ISCD-10) by polymerase chain reaction (PCR)-based identification of Neisseria meningitidis (crgA), Streptococcus pneumoniae (ply) and Haemophilus influenzae (bexA) in cerebrospinal fluid samples. The multiplex PCR detected N. meningitidis in 92%, S. pneumoniae in 4% and H. influenzae in 1% of the 192 clinical samples assayed; 3% were negative for all three DNA targets. Bacterial DNA detection was found to be a valuable adjunct to enhance bacterial meningitis surveillance when the yield of specimens by culture is reduced. The implementation of PCR assays as a diagnostic procedure in Public Health Laboratories is perceived to be a significant advance in the investigation of bacterial meningitis. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Grupo de Doenças Virais, Rickettsioses e Febres Hemorrágicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Grupo de Doenças Virais, Rickettsioses e Febres Hemorrágicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | meningitis | pt_BR |
| Subject | PCR | pt_BR |
| Subject | Streptococcus pneumoniae | pt_BR |
| Subject | Neisseria meningitidis | pt_BR |
| Subject | Haemophilus influenzae | pt_BR |
| Subject | cerebrospinal fluid | pt_BR |
| e-ISSN | 1678-8060 | |
