| Author | Dantas-Torres, Filipe | |
| Author | Martins, Thiago F. | |
| Author | Paiva-Cavalcanti, Milena de | |
| Author | Figueredo, Luciana A. | |
| Author | Lima, Bruna S. | |
| Author | Brandão-Filho, Sinval P. | |
| Access date | 2018-08-29T13:30:41Z | |
| Available date | 2018-08-29T13:30:41Z | |
| Document date | 2010 | |
| Citation | DANTAS-TORRES, F. et al. Transovarial passage of Leishmania infantum kDNA in artificially infected Rhipicephalus sanguineus. Experimental Parasitology, v. 125, n. 2, p. 184–185, jun. 2010. | pt_BR |
| ISSN | 1090-2449 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28427 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Carrapatos | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Transmissão Transovarial | pt_BR |
| Title | Transovarial passage of Leishmania infantum kDNA in artificially infected Rhipicephalus sanguineus | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.exppara.2010.02.003 | |
| Abstract | Phlebotomine sand flies are the only proven biological vectors of Leishmania parasites. However, Rhipicephalus sanguineus ticks have long been suspected to transmit Leishmania infantum in studies carried out in laboratory and natural conditions. In the present study, 5 microl of L. infantum promastigotes (1x10(6) cells per ml) was injected into the hemocel through the coxa I of four engorged females (F1, F2, F3 and F4). Control ticks (F5 and F6) were injected with sterile phosphate-buffered saline (PBS) using the same procedure. Then, these females, their eggs, and the originated larvae were tested by real time polymerase chain reaction (real-time PCR) for the presence of L. infantum kinetoplast DNA (kDNA). Females and eggs were tested after the end of the oviposition period (about 5 weeks post-inoculation) whereas larvae were tested about 4 months after the inoculation of females. All artificially infected females were positive for L. infantum kDNA. In addition, two pools of eggs (one from F2 and other from F4) and four pools of larvae (one from each F1 and F4 and two from F2) were positive for L. infantum kDNA. These results showed, for the first time, the transovarial passage of L. infantum kDNA in R. sanguineus ticks, thus suggesting that the transovarial transmission of L. infantum protozoa in ticks is worth to be investigated. | pt_BR |
| Affilliation | Università degli Studi di Bari. Dipartimento di Sanità Pubblica e Zootecnia. Valenzano, BA, Italy. | |
| Affilliation | Universidade de São Paulo. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo, SP, Brazil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | |
| Affilliation | Università degli Studi di Bari. Dipartimento di Sanità Pubblica e Zootecnia. Valenzano, BA, Italy. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | |
| Subject | Ticks | pt_BR |
| Subject | Leishmania infantum | pt_BR |
| Subject | Transovarial transmission | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Vetores de Aracnídeos / parasitologia | pt_BR |
| DeCS | DNA de Cinetoplasto / isolamento e purificação | pt_BR |
| DeCS | Fêmea | pt_BR |
| DeCS | Larva / parasitologia | pt_BR |
| DeCS | Leishmania infantum / genética | pt_BR |
| DeCS | Leishmania infantum / isolamento & purificação | pt_BR |
| DeCS | Leishmaniose Visceral / transmissão | pt_BR |
| DeCS | Fase de Clivagem do Zigoto / parasitologia | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | Rhipicephalus sanguineus / parasitologia | pt_BR |
| Embargo date | 2050-01-01 | |
