| Author | Assis, Andrêssa Silvino Ferreira | |
| Author | Miagostovich, Marize Pereira | |
| Author | Drumond, Betânia Paiva | |
| Author | Silva, Maria Luzia da Rosa e | |
| Access date | 2018-08-30T12:05:36Z | |
| Available date | 2018-08-30T12:05:36Z | |
| Document date | 2018 | |
| Citation | ASSIS, Andrêssa Silvino Ferreira; et al. Adenovirus and rotavirus recovery from a treated effluent through an optimized skimmed-milk flocculation method. Environmental Science and Pollution Research, v.25, p.17025–17032, 2018. | pt_BR |
| ISSN | 0944-1344 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28460 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Vírus entérico | pt_BR |
| Subject in Portuguese | Floculação Orgânica | pt_BR |
| Subject in Portuguese | Contaminação da água | pt_BR |
| Subject in Portuguese | controle interno | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Efluente tratado | pt_BR |
| Subject in Portuguese | Adenovírus | pt_BR |
| Subject in Portuguese | Rotavírus | pt_BR |
| Title | Adenovirus and rotavirus recovery from a treated effluent through an optimized skimmed-milk flocculation method | pt_BR |
| Type | Article | |
| DOI | 10.1007/s11356-018-1873-x | |
| Abstract | Sewage treatment may be insufficient for the complete removal of enteric viruses, such as human adenoviruses (HAdV) and group A rotavirus (RVA). The differences in the efficiency of the treatment methodologies used may interfere with the detection of these viruses. The objective of this study was to optimize a skimmed-milk flocculation technique for the recovery of HAdV and RVA in the samples of treated effluent. The treated effluent collected at the wastewater treatment plant (WWTP) was processed via four protocols including modifications in the initial centrifugation step and the final concentration of skimmed-milk. The viral load and recovery rate were determined by quantitative PCR TaqMan® System. The highest recovery rates of HAdV, RVA, and bacteriophage PP7 (internal control process) were obtained when the concentration of skimmed-milk was doubled and no centrifugation step was used for the sample clarification. The optimized protocol was assessed in a field study conducted with 24 treated effluent samples collected bi-monthly during 2015. HAdV and RVA were detected in 50.0% (12/24) and 33.3% (08/24) of the samples tested, respectively, throughout the year, without seasonal variation (p > 0.05). This study corroborates the use of the organic flocculation method for virus recovery in environmental samples with the adaptation of the protocols to different aquatic matrices. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Laboratório de Virologia. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Laboratório de Virologia. Juiz de Fora, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Laboratório de Virologia. Juiz de Fora, MG, Brasil. | pt_BR |
| Subject | Treated effluent | pt_BR |
| Subject | Water contamination | pt_BR |
| Subject | Organic flocculation | pt_BR |
| Subject | Enteric virus | pt_BR |
| Subject | Internal control | pt_BR |
| Subject | Quantitative PCR | pt_BR |
| Subject | Adenovirus | pt_BR |
| Subject | Rotavirus | pt_BR |
| e-ISSN | 1614-7499 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 06 Água potável e saneamento | |
