| Author | Melo-Santos, M. A. V. | |
| Author | Varjal-Melo, J. J. M. | |
| Author | Araújo, A. P. | |
| Author | Gomes, T. C. S. | |
| Author | Paiva, M. H. S. | |
| Author | Regis, L. N. | |
| Author | Furtado, A. F. | |
| Author | Magalhaes, T. | |
| Author | Macoris, M. L. G. | |
| Author | Andrighetti, M. T. M. | |
| Author | Ayres, C. F. J. | |
| Access date | 2018-08-31T12:30:57Z | |
| Available date | 2018-08-31T12:30:57Z | |
| Document date | 2010 | |
| Citation | MELO-SANTOS, M. A. V. et al. Resistance to the organophosphate temephos: mechanisms, evolution and reversion in an Aedes aegypti laboratory strain from Brazil. Acta Tropica, v. 113, n. 2, p. 180–189, fev. 2010. | pt_BR |
| ISSN | 1873-6254 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28489 | |
| Sponsorship | CNPq (bolsas 501581 / 2003-0 e 41.0542 / 2006-7 ) e FACEPE ( APQ-1361-4.00 / 08 ). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Mosquito | pt_BR |
| Subject in Portuguese | Resistência a inseticidas | pt_BR |
| Subject in Portuguese | Seleção laboratorial | pt_BR |
| Subject in Portuguese | Ensaio bioquímico | pt_BR |
| Subject in Portuguese | Resistência cruzada | pt_BR |
| Subject in Portuguese | Reversão de resistência | pt_BR |
| Title | Resistance to the organophosphate temephos: mechanisms, evolution and reversion in an Aedes aegypti laboratory strain from Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.actatropica.2009.10.015 | |
| Abstract | Insecticide resistance is one of the main problems in vector control programs. Because insects have developed resistance to all classes of available chemical insecticides, a proper surveillance and management of resistance in areas where these compounds are being utilized is crucial for the success of control programs. Since the mechanisms and molecular bases of resistance are various, they must be characterized to allow efficient monitoring strategies. Here we report the establishment of an Aedes aegypti strain resistant to temephos, named RecR, selected under laboratory conditions. The parental A. aegypti population was obtained from eggs collected in an area where temephos had been used for 8 years, and presented a baseline resistance ratio (RR) of 7. After 17 generations under selective pressure, the RR has increased to 180. Biochemical assays indicate that metabolic mechanisms are involved on temephos resistance in the selected strain. These experiments showed that, compared to the susceptible colony Rockefeller, RecR present higher activity of glutathione S-transferases (GSTs), alpha- and beta-esterases, and, to a lesser degree, mixed function oxidases (MFO). At the 14th or 17th generations, there was no cross resistance of these insects to deltamethrin, cypermethrin and malathion, while a low resistance level (RR=3) was observed for pyriproxyfen, a juvenile hormone analogue. Experiments on resistance reversal, performed through three different field simulated schemes using the resistant strain, showed that temephos susceptibility can be recovered. The establishment of an A. aegypti colony resistant to temephos is extremely valuable for a deeper understanding of resistance mechanisms and thus for further improvements in control strategies against this vector. With the urgent need on improving methodologies to monitor resistance, molecular studies such as microarrays, and resistant colonies such as RecR will certainly hasten such studies. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Departamento de Zoologia. Mestrado em Biologia Animal. Recife PE, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil / Associação Caruaruense de Ensino Superior (ASCES). Caruaru, PE, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Superintendencia de Controle de Endemias (SUCEN) Núcleo de Pesquisa/Serviço Regional 11 . Marília SP, Brazil. | pt_BR |
| Affilliation | Superintendencia de Controle de Endemias (SUCEN) Núcleo de Pesquisa/Serviço Regional 11 . Marília SP, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Subject | Mosquito | pt_BR |
| Subject | Insecticide resistance | pt_BR |
| Subject | Laboratory selection | pt_BR |
| Subject | Biochemical assay | pt_BR |
| Subject | Cross resistance | pt_BR |
| Subject | Resistance reversal | pt_BR |
| DeCS | Aedes / efeitos de drogas | pt_BR |
| DeCS | Aedes / genética | pt_BR |
| DeCS | Aedes / crescimento e desenvolvimento | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Animais, Laboratório | pt_BR |
| DeCS | Ensaio Biológico | pt_BR |
| DeCS | Brasil | pt_BR |
| DeCS | Evolução Molecular Dirigida | pt_BR |
| DeCS | Proteínas de Insetos / genética | pt_BR |
| DeCS | Proteínas de Insetos / metabolismo | pt_BR |
| DeCS | Vetores de insetos / efeitos de drogas | pt_BR |
| DeCS | Vetores de insetos / genética | pt_BR |
| DeCS | Resistência a inseticidas / genética | pt_BR |
| DeCS | Inseticidas / farmacologia | pt_BR |
| DeCS | Temefós / farmacologia | pt_BR |
| Embargo date | 2050-01-01 | |
