| Author | Lund, Ole | |
| Author | Nascimento, Eduardo J. M. | |
| Author | Maciel, Milton | |
| Author | Nielsen, Morten | |
| Author | Larsen, Mette Voldby | |
| Author | Lundegaard, Claus | |
| Author | Harndahl, Mikkel | |
| Author | Lamberth, Kasper | |
| Author | Buus, Søren | |
| Author | Salmon, Jérôme | |
| Author | August, Thomas J. | |
| Author | Marques, Ernesto T. A. | |
| Access date | 2018-09-11T12:09:38Z | |
| Available date | 2018-09-11T12:09:38Z | |
| Document date | 2011 | |
| Citation | LUND, O. et al. Human leukocyte antigen (HLA) class I restricted epitope discovery in yellow fewer and dengue viruses: importance of HLA binding strength. PloS One, v. 6, n. 10, p. e26494, 2011. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28623 | |
| Sponsorship | Este trabalho foi apoiado por contratos NIAID HHSN266200400083C, HHSN266200400025C ( http://www.niaid.nih.gov/ ). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Man-Seong Park, Faculdade de Medicina da Universidade de Hallym, República da Coréia | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Antígenos | pt_BR |
| Subject in Portuguese | Resposta imune | pt_BR |
| Subject in Portuguese | Imunoensaios ligados a enzimas | pt_BR |
| Subject in Portuguese | Células T | pt_BR |
| Subject in Portuguese | Vacinas | pt_BR |
| Title | Human leukocyte antigen (HLA) class I restricted epitope discovery in yellow fewer and dengue viruses: importance of HLA binding strength | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pone.0026494 | |
| Abstract | Epitopes from all available full-length sequences of yellow fever virus (YFV) and dengue fever virus (DENV) restricted by Human Leukocyte Antigen class I (HLA-I) alleles covering 12 HLA-I supertypes were predicted using the NetCTL algorithm. A subset of 179 predicted YFV and 158 predicted DENV epitopes were selected using the EpiSelect algorithm to allow for optimal coverage of viral strains. The selected predicted epitopes were synthesized and approximately 75% were found to bind the predicted restricting HLA molecule with an affinity, K(D), stronger than 500 nM. The immunogenicity of 25 HLA-A*02:01, 28 HLA-A*24:02 and 28 HLA-B*07:02 binding peptides was tested in three HLA-transgenic mice models and led to the identification of 17 HLA-A*02:01, 4 HLA-A*2402 and 4 HLA-B*07:02 immunogenic peptides. The immunogenic peptides bound HLA significantly stronger than the non-immunogenic peptides. All except one of the immunogenic peptides had K(D) below 100 nM and the peptides with K(D) below 5 nM were more likely to be immunogenic. In addition, all the immunogenic peptides that were identified as having a high functional avidity had K(D) below 20 nM. A*02:01 transgenic mice were also inoculated twice with the 17DD YFV vaccine strain. Three of the YFV A*02:01 restricted peptides activated T-cells from the infected mice in vitro. All three peptides that elicited responses had an HLA binding affinity of 2 nM or less. The results indicate the importance of the strength of HLA binding in shaping the immune response. | pt_BR |
| Affilliation | Technical University of Denmark. Center for Biological Sequence Analysis. Department of Systems Biology. Lyngby, Denmark. | pt_BR |
| Affilliation | University of Pittsburgh. Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburgh, Pennsylvania, United States of America. | pt_BR |
| Affilliation | Johns Hopkins University. School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, Maryland, United States of America. | pt_BR |
| Affilliation | Technical University of Denmark. Center for Biological Sequence Analysis. Department of Systems Biology. Lyngby, Denmark. | pt_BR |
| Affilliation | Technical University of Denmark. Center for Biological Sequence Analysis. Department of Systems Biology. Lyngby, Denmark. | pt_BR |
| Affilliation | Technical University of Denmark. Center for Biological Sequence Analysis. Department of Systems Biology. Lyngby, Denmark. | pt_BR |
| Affilliation | University of Copenhagen. The Panum Institute. Institute of Medical Microbiology and Immunology. Division of Experimental Immunology. Copenhagen, Denmark. | pt_BR |
| Affilliation | University of Copenhagen. The Panum Institute. Institute of Medical Microbiology and Immunology. Division of Experimental Immunology. Copenhagen, Denmark. | pt_BR |
| Affilliation | University of Copenhagen. The Panum Institute. Institute of Medical Microbiology and Immunology. Division of Experimental Immunology. Copenhagen, Denmark. | pt_BR |
| Affilliation | Johns Hopkins University. School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, Maryland, United States of America. | pt_BR |
| Affilliation | Johns Hopkins University. School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, Maryland, United States of America. | pt_BR |
| Affilliation | University of Pittsburgh. Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburgh, Pennsylvania, United States of America / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Subject | Antigens | pt_BR |
| Subject | Immune response | pt_BR |
| Subject | Enzyme-linked immunoassays | pt_BR |
| Subject | T cells | pt_BR |
| Subject | Vaccines | pt_BR |
| DeCS | Vírus da Dengue / imunologia | pt_BR |
| DeCS | Epitopos / imunologia | pt_BR |
| DeCS | Antígenos de Histocompatibilidade Classe I / imunologia | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Ratos transgênicos | pt_BR |
| DeCS | Dados de Sequencia Molecular | pt_BR |
| DeCS | Vacina contra Febre Amarela / imunologia | pt_BR |
| DeCS | Vírus da febre amarela / imunologia | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
