| Author | Galvão, Cleber Eduardo | |
| Author | Fragoso, Stenio Perdigão | |
| Author | Oliveira, Carina Elisei de | |
| Author | Forner, Odinéia | |
| Author | Pereira, Renata Ribeiro Bastos | |
| Author | Soares, Cleber Oliveira | |
| Author | Rosinha, Grácia Maria Soares | |
| Access date | 2018-09-12T15:14:52Z | |
| Available date | 2018-09-12T15:14:52Z | |
| Document date | 2017 | |
| Citation | GALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017. | pt_BR |
| ISSN | 1471-2180 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28690 | |
| Language | por | pt_BR |
| Publisher | Springer Nature | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Corynebacterium pseudotuberculosis | pt_BR |
| Title | Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/s12866-017-1110-7 | |
| Abstract | Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA.
For this, a wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli. For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. This way, concluded that, proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. | pt_BR |
| Affilliation | Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Católica Dom Bosco. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil. | pt_BR |
| Subject | Lymphadenitis | pt_BR |
| Subject | Gene Expression | pt_BR |
| Subject | Gene Library | pt_BR |
| Subject | Vaccines | pt_BR |
| Subject in Spanish | Linfadenitis | pt_BR |
| Subject in Spanish | Expresión Génica | pt_BR |
| Subject in Spanish | Biblioteca de Genes | pt_BR |
| Subject in Spanish | Vacunas de ADN | pt_BR |
| DeCS | Linfadenite | pt_BR |
| DeCS | Expressão Gênica | pt_BR |
| DeCS | Biblioteca Gênica | pt_BR |
| DeCS | Vacinas de DNA | pt_BR |
| e-ISSN | 1471-2180 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
