| Author | Queiroz, Rafaella Fortini Grenfell e | |
| Author | Taboada, Diana | |
| Author | Coutinho, Lucélia Antunes | |
| Author | Pedrosa, Maria Luysa de Camargos | |
| Author | Assis, Jéssica Vieira | |
| Author | Pinto, Matheus de Oliveira Silva | |
| Author | Cruz, Renata Ramalho | |
| Author | Oliveira, Áureo Almeida de | |
| Author | Moraes, Vanessa Silva | |
| Author | Katz, Naftale | |
| Author | Coelho, Paulo Marcos Zech | |
| Access date | 2018-09-12T17:03:34Z | |
| Available date | 2018-09-12T17:03:34Z | |
| Document date | 2018 | |
| Citation | QUEIROZ, Rafaella Fortini Grenfell e et al. Innovative methodology for point-of-care circulating cathodic antigen with rapid urine concentration for use in the field for detecting low Schistosoma mansoni infection and for control of cure with high accuracy. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 112, n. 1, p. 1-7, 2018 | pt_BR |
| ISSN | 0035-9203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28693 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Controle de cura | pt_BR |
| Subject in Portuguese | Reatividade cruzada | pt_BR |
| Subject in Portuguese | Concentração rápida de urina | pt_BR |
| Subject in Portuguese | Melhoria POC-CCA | pt_BR |
| Subject in Portuguese | Esquistossomose mansoni | pt_BR |
| Subject in Portuguese | Definição de leituras de traços | pt_BR |
| Title | Innovative methodology for point-of-care circulating cathodic antigen with rapid urine concentration for use in the field for detecting low Schistosoma mansoni infection and for control of cure with high accuracy | pt_BR |
| Type | Article | |
| DOI | 10.1093/trstmh/try014 | |
| Abstract | Background: Prior to eliminating schistosomiasis, efforts must address accurate and fast individual diagnosis.
Diagnosis is still inaccurate by parasitological and point-of-care circulating cathodic antigen (POC-CCA) inareas of low endemicity.
Methods: Our group has optimized POC-CCA with a 30 min urine concentration step with no need for specialized technicians or equipment and with high accuracy. We evaluated this new method, called POC-CCA filter (FLT), in two Brazilian endemic areas with distinct profiles.
Results: At baseline, POC-CCA had a poor performance with several false results and undefined trace readings, revealing a prevalence rate of 10% against a rate of 23% for POC-CCA FLT, which was similar to the parasitological rates. Accuracy increased from as low as 0.36 to 0.96 after urine concentration in one area. POC-CCA properly diagnosed only half of the cases at three post-treatment time points, while POC-CCA FLT was able to diagnose 96, 83 and 100%, respectively.
Conclusions: The improvement of conventional POC methodology by a fast and simple urine concentration step provided not only an increase in its accuracy before and after praziquantel treatment, but also preserved its applicability in low-prevalence endemic areas, allowing the definition of trace readings as negative cases | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil/Department of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, Georgia, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Control of cure | pt_BR |
| Subject | Cross-reactivity | pt_BR |
| Subject | Fast urine concentration | pt_BR |
| Subject | POC-CCA improvement | pt_BR |
| Subject | Schistosomiasis mansoni | pt_BR |
| Subject | Trace readings definition | pt_BR |
| Embargo date | 2025-01-01 | |
