| Author | Fonseca, Bruna P. F. | pt_BR |
| Author | Marques, Christiane F. S. | pt_BR |
| Author | Nascimento, Lílian D. | pt_BR |
| Author | Mello, Marcelle B. | pt_BR |
| Author | Silva, Leila B. R. | pt_BR |
| Author | Rubim, Nara M. | pt_BR |
| Author | Foti, Leonardo | pt_BR |
| Author | Silva, Edimilson Domingos da | pt_BR |
| Author | Ferreira, Antônio Gomes Pinto | pt_BR |
| Author | Krieger, Marco Aurélio | pt_BR |
| Access date | 2018-09-13T14:21:41Z | |
| Available date | 2018-09-13T14:21:41Z | |
| Document date | 2011 | |
| Citation | FONSECA, Bruna P. F. et al. Development of a multiplex bead-based assay for detection of Hepatitis C virus. Clinical and Vaccine Immunology, v. 18, n. 5, p. 802-806, 3 May 2011. | pt_BR |
| ISSN | 1556-6811 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28739 | |
| Language | por | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Hepacivirus | pt_BR |
| Title | Development of a multiplex bead-based assay for detection of Hepatitis C virus | en_US |
| Type | Article | pt_BR |
| DOI | 10.1128/CVI.00265-10 | pt_BR |
| Abstract | Hepatitis C virus (HCV) infection is a major burden to public health worldwide, affecting approximately 3% of the human population. Although HCV detection is currently based on reliable tests, the field of medical diagnostics has a growing need for inexpensive, accurate, and quick high-throughput assays. By using the recombinant HCV antigens NS3, NS4, NS5, and Combined, we describe a new bead-based multiplex test capable of detecting HCV infection in human serum samples. The first analysis, made in a singleplex format, showed that each antigen coupled to an individual bead set presented high-level responses for anti-HCV-positive reference serum pools and lower-level responses for the HCV-negative pools. Our next approach was to determine the sensitivity and specificity of each antigen by testing 93 HCV-positive and 93 HCV-negative sera. When assayed in the singleplex format, the NS3, NS4, and NS5 antigens presented lower sensitivity values (50.5%, 51.6%, and 55.9%, respectively) than did the Combined antigen, which presented a sensitivity of 93.5%. All antigens presented 100% specificity. These antigens were then multiplexed in a 4-plex assay, which resulted in increased sensitivity and specificity values, performing with 100% sensitivity and 100% specificity. The positive and negative predictive values for the 4-plex assay were 100%. Although preliminary, this 4-plex assay showed robust results that, aligned with its small-sample-volume requirements and also its cost- and time-effectiveness, make it a reasonable alternative to tests currently used for HCV screening of potentially infected individuals. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
| Subject | Hepatitis C | en_US |
| Subject | Hepatitis Antigens | en_US |
| Subject | Reagent Kits | en_US |
| Subject | Diagnostic | en_US |
| Subject in Spanish | Antígenos de la Hepatitis | es_ES |
| Subject in Spanish | Juego de Reactivos para Diagnóstico | es_ES |
| DeCS | Hepatite C | pt_BR |
| DeCS | Antígenos de Hepatite | pt_BR |
| DeCS | Kit de Reagentes para Diagnóstico | pt_BR |
| e-ISSN | 1556-679X | pt_BR |
