| Author | Medeiros, Maria das Graças Freire de | |
| Author | Silva, Aline Caroline da | |
| Author | Citó, Antonia Maria das Graças Lopes | |
| Author | Borges, Andrezza Raposo | |
| Author | Lima, Sidney Gonçalo de | |
| Author | Lopes, José Arimatéia Dantas | |
| Author | Figueiredo, Regina Celia Bressan Queiroz | |
| Access date | 2018-09-14T11:56:40Z | |
| Available date | 2018-09-14T11:56:40Z | |
| Document date | 2011 | |
| Citation | MEDEIROS, M. das G. F. de et al. In vitro antileishmanial activity and cytotoxicity of essential oil from Lippia sidoides Cham. Parasitology International, v. 60, n. 3, p. 237–241, set. 2011. | pt_BR |
| ISSN | 1873-0329 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28779 | |
| Sponsorship | CNPq , CAPES , UFPI , RENORBIO , FACEPE e FIOCRUZ | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Lippia sidoides | pt_BR |
| Subject in Portuguese | Óleo essencial | pt_BR |
| Subject in Portuguese | Atividade antileishmanial Leishmania amazonensis | pt_BR |
| Subject in Portuguese | Ultraestrutura | pt_BR |
| Title | In vitro antileishmanial activity and cytotoxicity of essential oil from Lippia sidoides Cham | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.parint.2011.03.004 | |
| Abstract | Leishmaniasis is a widespread tropical infection caused by different species of Leishmania protozoa. There is no vaccine available for Leishmania infections and conventional treatments are very toxic to the patients. Therefore, antileishmanial drugs are urgently needed. In this study we have analyzed the effects of essential oils from Lippia sidoides (LSEO) and its major compound thymol on the growth, viability and ultrastructure of Leishmania amazonensis. The essential oil and thymol showed significant activity against promastigote forms of L. amazonensis, with IC(50)/48 h of 44.38 and 19.47 μg/mL respectively. However, thymol showed toxicity against peritoneal macrophages and low selectivity against the promastigotes when compared with the crude LSEO. On the other hand, no cytotoxic effect was observed in macrophages treated with the crude essential oil. Incubation of L. amazonensis-infected macrophages with LSEO showed a marked reduction in amastigote survival within the macrophages. Significant morphological alterations as accumulation of large lipid droplets in the cytoplasm, disrupted membrane and wrinkled cells were usually seen in treated parasites. The LSEO's activity against both promastigote and the amstigote forms of L. amazonensis, together with its low toxicity to mammalian cells, point to LSEO as a promising agent for the treatment of cutaneous leishmaniasis. | pt_BR |
| Affilliation | Universidade Federal do Piauí. Programa de Pós-graduação em Biotecnologia-RENORBIO. Piauí, Brazil / Universidade Federal do Piauí. Campus Universitário Ministro Petrônio Portela-Ininga. Departamento de Química. Teresina, PI, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brazil. | pt_BR |
| Affilliation | Universidade Federal do Piauí. Campus Universitário Ministro Petrônio Portela-Ininga. Departamento de Química. Teresina, PI, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brazil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Programa de Pós-graduação em Saúde Pública. Recife, PE, Brazil. | pt_BR |
| Affilliation | Universidade Federal do Piauí. Programa de Pós-graduação em Biotecnologia-RENORBIO. Piauí, Brazil. | pt_BR |
| Affilliation | Universidade Federal do Piauí. Campus Universitário Ministro Petrônio Portela-Ininga. Departamento de Química. Teresina, PI, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brazil. | pt_BR |
| Subject | Lippia sidoides | pt_BR |
| Subject | Essential oil | pt_BR |
| Subject | Antileishmanial activity Leishmania amazonensis | pt_BR |
| Subject | Ultrastructure | pt_BR |
| DeCS | Agentes Antiprotozoários / isolamento & purificação | pt_BR |
| DeCS | Agentes Antiprotozoários / Farmacologia | pt_BR |
| DeCS | Concentração Inibidora 50 | pt_BR |
| DeCS | Leishmania / efeitos de drogas | pt_BR |
| DeCS | Leishmania / ultraestrutura | pt_BR |
| DeCS | Leishmaniose / quimioterapia | pt_BR |
| DeCS | Lippia / química | pt_BR |
| DeCS | Macrófagos Peritoneais / efeitos de drogas | pt_BR |
| DeCS | Óleos Voláteis / Farmacêuticos | pt_BR |
| DeCS | Testes de Sensibilidade Parasitária | pt_BR |
| DeCS | Extratos Vegetais / Farmacologia | pt_BR |
| DeCS | Timol / isolamento e purificação | pt_BR |
| DeCS | Ratos, endogâmicos BALB C | pt_BR |
| DeCS | Óleos voláteis / química | pt_BR |
| Embargo date | 2050-01-01 | |
