Author | Campos, Keillen Monick Martins | |
Author | Kuehn, Andrea | |
Author | Almeida, Anne | |
Author | Duarte, Ana Paula M. | |
Author | Sampaio, Vanderson S. | |
Author | Rodriguez, Íria C. | |
Author | Silva, Sara G. M. da | |
Author | Ríos-Velásquez, Claudia María | |
Author | Lima, José Bento Pereira | |
Author | Pimenta, Paulo Filemon Paolucci | |
Author | Bassat, Quique | |
Author | Müller, Ivo | |
Author | Lacerda, Marcus | |
Author | Monteiro, Wuelton M. | |
Author | Guerra, Maria das Graças Vale Barbosa | |
Access date | 2018-09-18T12:44:29Z | |
Available date | 2018-09-18T12:44:29Z | |
Document date | 2018 | |
Citation | CAMPOS, Keillen Monick Martins et al. Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon. Parasites and Vectors, v. 11, 288, 2018 | pt_BR |
ISSN | 1756-3305 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28849 | |
Language | eng | pt_BR |
Publisher | Springer Nature | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Malaria | pt_BR |
Subject in Portuguese | gamatocitos | pt_BR |
Subject in Portuguese | vetor | pt_BR |
Subject in Portuguese | eliminação | pt_BR |
Subject in Portuguese | Ensaio de alimentação de membrana | pt_BR |
Title | Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s13071-018-2749-0 | |
Abstract | Background: Asymptomatic individuals are one of the major challenges for malaria elimination programs in endemic areas. In the absence of clinical symptoms and with a lower parasite density they constitute silent reservoirs considered important for maintaining transmission of human malaria. Studies from Brazil have shown that infected individuals may carry these parasites for long periods.
Results: Patients were selected from three periurban endemic areas of the city of Manaus, in the western Brazilian Amazon. Symptomatic and asymptomatic patients with positive thick blood smear and quantitative real-time PCR (qPCR) positive for Plasmodium vivax were invited to participate in the study. A standardised pvs25 gene amplification by qPCR was used for P. vivax gametocytes detection. Anopheles aquasalis were fed using membrane feeding assays (MFA) containing blood from malaria patients. Parasitemia of 42 symptomatic and 25 asymptomatic individuals was determined by microscopic examination of blood smears and qPCR. Parasitemia density and gametocyte density were assessed as determinants of infection rates and oocysts densities. A strong correlation between gametocyte densities (microscopy and molecular techniques) and mosquito infectivity (P < 0.001) and oocysts median numbers (P < 0.05) was found in both groups. The ability to infect mosquitoes was higher in the symptomatic group (41%), but infectivity in the asymptomatic group was also seen (1.42%).
Conclusions: Although their infectivity to mosquitoes is relatively low, given the high prevalence of P. vivax asymptomatic carriers they are likely to play and important role in malaria transmission in the city of Manaus. The role of asymptomatic infections therefore needs to be considered in future malaria elimination programs in Brazil. | pt_BR |
Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Programa de Pós Graduação em Medicina Tropical. Manaus, AM, Brazil/Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil8/ISGlobal, Barcelona Centre for International Health Research. Hospital Clínic-Universitat de Barcelona, Barcelona, Spain. | pt_BR |
Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Programa de Pós Graduação em Medicina Tropical. Manaus, AM, Brazil/Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil | pt_BR |
Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Programa de Pós Graduação em Medicina Tropical. Manaus, AM, Brazil/Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil | pt_BR |
Affilliation | Fundação de Vigilância em Saúde. Manaus, Amazonas, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas Leônidas & Maria Deane, Manaus, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | ISGlobal, Barcelona Centre forInternational Health Research. Hospital Clínic-Universitat de Barcelona, Barcelona, Spain | pt_BR |
Affilliation | ISGlobal, Barcelona Centre forInternational Health Research. Hospital Clínic-Universitat de Barcelona, Barcelona, Spain/Walter and Eliza Hall Institute. Parkville, Australia. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto de Pesquisas Leônidas & Maria Deane, Manaus, Brazil. | pt_BR |
Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Programa de Pós Graduação em Medicina Tropical. Manaus, AM, Brazil/Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil | pt_BR |
Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Programa de Pós Graduação em Medicina Tropical. Manaus, AM, Brazil/Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil | pt_BR |
Subject | Malaria | pt_BR |
Subject | Gametocytes | pt_BR |
Subject | Vector | pt_BR |
Subject | Elimination | pt_BR |
Embargo date | 2024-01-01 | |
xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |