| Author | Oliveira, Tainara Santos | |
| Author | Varjão, Luana Milen | |
| Author | Silva, Lis Nery Nunes da | |
| Author | Pereira, Rodrigo de Castro Lisboa | |
| Author | Hofer, Ernesto | |
| Author | Vallim, Deyse Cristina | |
| Author | Almeida, Rogeria Comastri de Castro | |
| Access date | 2018-09-18T13:19:42Z | |
| Available date | 2018-09-18T13:19:42Z | |
| Document date | 2018 | |
| Citation | OLIVEIRA, Tainara Santos et al. Listeria monocytogenes at chicken slaughterhouse: Occurrence, genetic relationship among isolates and evaluation of antimicrobial susceptibility. Food Control, v. 88, p. 131-138, Jan. 2018. | pt_BR |
| ISSN | 0956-7135 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28853 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Listeria monocytogenes | pt_BR |
| Subject in Portuguese | Virulência | pt_BR |
| Subject in Portuguese | Matadouros | pt_BR |
| Subject in Portuguese | Suscetibilidade antimicrobiana | pt_BR |
| Title | Listeria monocytogenes at chicken slaughterhouse: Occurrence, genetic relationship among isolates and evaluation of antimicrobial susceptibility | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.foodcont.2018.01.015 | |
| Abstract | The aims of this study were to verify the occurrence of Listeria monocytogenes in chicken at industrial
slaughterhouse, the presence of virulence genes and genetic relationship among isolates, and evaluate
the antimicrobial susceptibility. A total of 195 samples distributed in 36 carcasses after evisceration, 36
after chilling and 36 after packing, 43 carcasses cutting and 44 cuts after packaging, were collected.
L. monocytogenes was found in 35 (17.9%) samples, especially in meat cuts, and samples collected after
packing were the ones with higher rate of contamination (8.3%). Serotyping revealed the presence of
three serovars: 1/2a (87%), 1/2c (8%) and 1/2b (5%). Macrorestriction profile with Apal at Pulsed-field gel
electrophoresis (PFGE) showed 12 pulsotypes, and isolates from carcass and drumettes after packing and
breast filet from cuts section showed high pulsotypes diversity. Regarding the profile of antimicrobial
susceptibility, 100% of the isolates were sensitive to most antibiotics tested, except for clindamycin; 42%
had intermediate resistance and 5% resistance. Isolation of L. monocytogenes in high levels in the chicken
slaughtering line, as found in this study points to a potential risk associated, because the pathogen remains
viable in the food throughout the cold chain that is used to commercialization. Still, the antimicrobial
susceptibility monitoring is of interest of public health and our result shows that the isolates were
susceptible to almost all antibiotics tested, indicating that treatment commonly used to control listeriosis
infection is adequate. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Escola de Nutrição. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Escola de Nutrição. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Escola de Nutrição. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacterianas. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacterianas. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Escola de Nutrição. Salvador, BA, Brasil. | pt_BR |
| Subject | Listeria monocytogenes | pt_BR |
| Subject | Virulence | pt_BR |
| Subject | PEGE | pt_BR |
| Subject | Antimicrobial susceptibility | pt_BR |
| Subject | Slaughterhouse | pt_BR |
| Embargo date | 2030-01-01 | |
