| Author | Ribeiro, Kleber Silva | |
| Author | Vasconcellos, Camilla Ioshida | |
| Author | Soares, Rodrigo Pedro Pinto | |
| Author | Mendes, Maria Tays | |
| Author | Ellis, Cameron C. | |
| Author | Aguilera-Flores, Marcela | |
| Author | Almeida, Igor Correia de | |
| Author | Schenkman, Sergio | |
| Author | Iwai, Leo Kei | |
| Author | Torrecilhas, Ana Claudia | |
| Access date | 2018-09-18T14:11:11Z | |
| Available date | 2018-09-18T14:11:11Z | |
| Document date | 2018 | |
| Citation | RIBEIRO, Kleber Silva et al. Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells. J Extracell Vesicles., v. 7, n. 1, 1463779, 2018. | pt_BR |
| ISSN | 2001-3078 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28860 | |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Vesículas extracelulares | pt_BR |
| Subject in Portuguese | T. cruzi | pt_BR |
| Subject in Portuguese | Interação parasita-hospedeiro | pt_BR |
| Title | Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells | pt_BR |
| Type | Article | |
| DOI | 10.1080/20013078.2018.1463779 | |
| Abstract | Trypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy. EVs were purified by exclusion chromatography or ultracentrifugation and quantitated using nanoparticle tracking analysis. Trypomastigotes from YuYu strain released higher number of EVs than those from Y strain, enriched with virulence factors trans-sialidase (TS) and cruzipain. Proteomic analysis confirmed the increased abundance of proteins coded by the TS gene family, mucin-like glycoproteins, and some typical exosomal proteins in the YuYu strain, which also showed considerable differences between purified EVs and vesicle-free fraction as compared to the Y strain. To evaluate whether such differences were related to parasite infectivity, J774 macrophages and LLC-MK2 kidney cells were preincubated with purified EVs from both strains and then infected with Y strain trypomastigotes. EVs released by YuYu strain caused a lower infection but higher intracellular proliferation in J774 macrophages than EVs from Y strain. In contrast, YuYu strain-derived EVs caused higher infection of LLC-MK2 cells than Y strain-derived EVs. In conclusion, quantitative and qualitative differences in EVs and secreted proteins from different T. cruzi strains may correlate with infectivity/virulence during the host–parasite interaction. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Diadema, SP, Brazil | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Diadema, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Border Biomedical Research Center. Department of Biological Sciences. University of Texas at El Paso. El Paso, TX, USA | pt_BR |
| Affilliation | Border Biomedical Research Center. Department of Biological Sciences. University of Texas at El Paso. El Paso, TX, USA | pt_BR |
| Affilliation | Border Biomedical Research Center. Department of Biological Sciences. University of Texas at El Paso. El Paso, TX, USA | pt_BR |
| Affilliation | Border Biomedical Research Center. Department of Biological Sciences. University of Texas at El Paso. El Paso, TX, USA | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Instituto Butantan. Centro de Toxinas, Resposta Imunitária e Sinalização Celular. Laboratório Especial de Toxicologia Aplicada. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Ciências Farmacêuticas. Diadema, SP, Brazil | pt_BR |
| Subject | Extracellular vesicles | pt_BR |
| Subject | T. cruzi | pt_BR |
| Subject | host interaction | pt_BR |
| Embargo date | 2022-01-01 | |
