Author | Espada, Caroline Ricce | |
Author | Ortiz, Paola Andrea | |
Author | Shaw, Jeffrey Jon | |
Author | Barral, Aldina Maria Prado | |
Author | Costa, Jackson Mauricio Lopes | |
Author | Uliana, Silvia Reni Bortolin | |
Author | Coelho, Adriano Cappellazzo | |
Access date | 2018-09-19T16:47:03Z | |
Available date | 2018-09-19T16:47:03Z | |
Document date | 2018 | |
Citation | ESPADA, C. R. et al. Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations. Diagnostic Microbiology and Infectious Disease, v. 91, p. 312–318, 2018. | pt_BR |
ISSN | 0732-8893 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28899 | |
Sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2011/20484-7 and 2015/09080-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 473343/2012-6), Brazil. SRBU is the
recipient of a senior researcher scholarship from CNPq. ACC and CRE were fellows supported by FAPESP (2012/14629-5 and 2016/23405-4). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania (Leishmania) amazonensis | pt_BR |
Subject in Portuguese | Espécie Leishmania (Viannia) | pt_BR |
Subject in Portuguese | hsp70 | pt_BR |
Subject in Portuguese | PCR-RFLP | pt_BR |
Subject in Portuguese | Diagnóstico molecular | pt_BR |
Title | Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.diagmicrobio.2018.03.004 | |
Abstract | Hsp70 is a cytoplasmic heat-shock protein, encoded by a multicopy tandemly repeated gene that has recently been gaining popularity as a valuable marker for typing Leishmania species. In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L. (L.) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L. (Viannia) naiffi strains. The profiles of both L. (V.) shawi and L. (V.) lindenbergi were very similar to those of other L. (Viannia) species. The observations described herein reflect the polymorphism found within species of Leishmania and indicate that results from this hsp70 PCR-RFLP method should be used with caution when typing isolates from clinical cases of leishmaniasis and Leishmania species from Brazil. | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil | pt_BR |
Subject | Leishmania (Leishmania) amazonensis | pt_BR |
Subject | Leishmania (Viannia) species | pt_BR |
Subject | hsp70 | pt_BR |
Subject | PCR-RFLP | pt_BR |
Subject | Molecular diagnosis | pt_BR |